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Product Info Summary
| SKU: | EKC1359 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
MEF2A Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1359
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The MEF2A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MEF2A protein expression profile in cells. The kit can be used for measuring the relative amounts of MEF2A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MEF2A.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
MEF2A Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1359)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1359 is reactive to MEF2A in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1359 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-MEF2A Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HeLa cells
Protein Target Info & Infographic
Gene/Protein Information For MEF2A (Source: Uniprot.Org, NCBI)
Gene Name
MEF2A
Full Name
Myocyte-specific enhancer factor 2A
Weight
54811 MW
Superfamily
MEF2 family
Alternative Names
MEF2;Myocyte-specific enhancer factor 2A;Serum response factor-like protein 1 MEF2A ADCAD1, RSRFC4, RSRFC9, mef2 myocyte enhancer factor 2A myocyte-specific enhancer factor 2A|MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)|serum response factor-like protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MEF2A, check out the MEF2A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MEF2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MEF2A Colorimetric Cell-Based ELISA Kit (EKC1359)
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