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Product Info Summary
| SKU: | LS112950 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of mediator complex subunit 8 (MED8), transcript variant 1 |
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Product info
Product Name
MED8 (NM_201542) Human Over-expression Lysate
SKU/Catalog Number
LS112950
Size
100 µg
Description
Transient overexpression lysate of mediator complex subunit 8 (MED8), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
MED8 (NM_201542) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS112950)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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MED8 Human Over-expression Lysates NM_001001654.
Western blot validation of overexpression lysate (LS112950) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MED8 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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MED8 Human Over-expression Lysates NM_052877.
Western blot validation of overexpression lysate (LS112950) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MED8 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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MED8 Human Over-expression Lysates NM_201542.
Western blot validation of overexpression lysate (LS112950) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MED8 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for MED8 (Source: Uniprot.org, NCBI)
Gene Name
MED8
Full Name
Mediator of RNA polymerase II transcription subunit 8
Weight
29.199kDa
Superfamily
Mediator complex subunit 8 family
Alternative Names
ARC32subunit 8 homolog; mediator complex subunit 8mediator of RNA polymerase II transcription, subunit 8 homolog (S. cerevisiae)
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MED8, check out the MED8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MED8 (NM_201542) Human Over-expression Lysate (LS112950)
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