MAP4K6 Colorimetric Cell-Based ELISA

Product Name

MAP4K6 Colorimetric Cell-Based ELISA

See all MAP4K6 products

SKU/Catalog Number

EKC1871

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The MAP4K6 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MAP4K6 protein expression profile in cells. The kit can be used for measuring the relative amounts of MAP4K6 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MAP4K6.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

MAP4K6 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1871)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1871 is reactive to MINK1 B55, MAP4K6 , MINK , YSK2, ZC3 in Human, Mouse samples

Application Guarantee

EKC1871 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent	Quantity	Container
96-Well Cell Culture Clear-Bottom Microplate	1 Plates	-
10x TBS	24 ml (10x)	Clear
Quenching Buffer	24 ml (1x)	Clear
Blocking Buffer	50 ml (1x)	Clear
15x Wash Buffer	50 ml (15x)	Clear
100x Anti-MAP4K6 Antibody (Rabbit Polyclonal)	60 µl (100x)	Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)	60 µl (100x)	Green
HRP-Conjugated Anti-Rabbit IgG Antibody	6ml (1x)	Glass
HRP-Conjugated Anti-Mouse IgG Antibody	6ml (1x)	Glass
Primary Antibody Diluent	12 ml (1x)	Clear
Ready-to-Use Substrate	12 ml (1x)	Brown
Stop Solution	12 ml (1x)	Clear
Crystal Violet Solution	6ml (1x)	Glass
SDS Solution	24 ml (1x)	Clear
Adhesive Plate Seals	2 Seals	-

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
• Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
• 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
• Deionized or sterile water
• Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
• Graph paper or computer software capable of generating or displaying logarithmic functions
• Absorbent papers or vacuum aspirator
• Test tubes or microfuge tubes capable of storing 1 ml
• Orbital shaker
• Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For MINK1 B55, MAP4K6 , MINK , YSK2, ZC3 (Source: Uniprot.Org, NCBI)

Alternative Names

Misshapen-like kinase 1;2.7.11.1;GCK family kinase MiNK;MAPK/ERK kinase kinase kinase 6;MEK kinase kinase 6;MEKKK 6;Misshapen/NIK-related kinase;Mitogen-activated protein kinase kinase kinase kinase 6;MINK1 ;B55, MAP4K6 , MINK , YSK2, ZC3; MINK1 B55, MAP4K6, MINK, YSK2, ZC3 misshapen like kinase 1 misshapen-like kinase 1|GCK family kinase MINK|MAPK/ERK kinase kinase kinase 6|MEK kinase kinase 6|MEKKK 6|misshapen/NIK-related kinase|mitogen-activated protein kinase kinase kinase kinase 6

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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