Product Info Summary
SKU: | A07952 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-CREBZF/Zhangfei Antibody
SKU/Catalog Number
A07952
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CREBZF/Zhangfei Antibody (Catalog # A07952). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CREBZF/Zhangfei Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07952)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CREBZF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07952 is reactive to CREBZF in Human, Mouse
Reconstitution
Calculated molecular weight
37134 MW
Background of Zhangfei
Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.
Lu R., Nucleic Acids Res. 28:2446-2454(2000).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Misra V., J. Biol. Chem. 280:15257-15266(2005).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07952 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from RAW264.7 cells, using CREBZF antibody A07952.
Click image to see more details
Immunofluorescence analysis of HepG2 cells, using CREBZF antibody A07952.
Protein Target Info & Infographic
Gene/Protein Information For CREBZF (Source: Uniprot.org, NCBI)
Gene Name
CREBZF
Full Name
CREB/ATF bZIP transcription factor
Weight
37134 MW
Superfamily
bZIP family
Alternative Names
CREB/ATF bZIP transcription factor;Host cell factor-binding transcription factor Zhangfei;HCF-binding transcription factor Zhangfei;CREBZF;ZF; CREBZF SMILE, ZF CREB/ATF bZIP transcription factor CREB/ATF bZIP transcription factor|HCF-binding transcription factor Zhangfei|SHP-interacting leucine zipper protein|Zhangfei|host cell factor-binding transcription factor Zhangfei
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CREBZF, check out the CREBZF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CREBZF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CREBZF/Zhangfei Antibody (A07952)
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No publications found for A07952
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Customer Q&As
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4 Customer Q&As for Anti-CREBZF/Zhangfei Antibody
Question
I see that the anti-CREBZF/Zhangfei antibody A07952 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-21
Question
We are currently using anti-CREBZF/Zhangfei antibody A07952 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-CREBZF/Zhangfei antibody (A07952) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-CREBZF/Zhangfei antibody A07952. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Will anti-CREBZF/Zhangfei antibody A07952 work for IF with bone marrow?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
According to the expression profile of bone marrow, CREBZF is highly expressed in bone marrow. So, it is likely that anti-CREBZF/Zhangfei antibody A07952 will work for IF with bone marrow.
Boster Scientific Support
Answered: 2018-01-09