Product Info Summary
SKU: | EKC1886 |
---|---|
Size: | 1 kit, containing one 96-well plate and all necessary reagents |
Reactive Species: | Human, Mouse, Rat |
Application: | ELISA |
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Product info
Product Name
MADD Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1886
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The MADD Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MADD protein expression profile in cells. The kit can be used for measuring the relative amounts of MADD in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MADD.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
MADD Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1886)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1886 is reactive to MADD in Human, Mouse, Rat samples
Application Guarantee
EKC1886 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-MADD Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 6ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
![boster box boster box](https://www.bosterbio.com/media/catalog/product/b/o/boster-box.png)
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Boster Kit Box
![ekc1886 madd colorimetric cell based elisa wb testing 1 ekc1886 madd colorimetric cell based elisa wb testing 1](https://www.bosterbio.com/media/catalog/product/e/k/ekc1886-madd-colorimetric-cell-based-elisa-wb-testing-1.jpg)
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Western blot analysis of extracts from COLO/HeLa/Jurkat cells
Protein Target Info & Infographic
Gene/Protein Information For MADD (Source: Uniprot.Org, NCBI)
Gene Name
MADD
Full Name
MAP kinase-activating death domain protein
Weight
183303 MW
Superfamily
MADD family
Alternative Names
MAP kinase-activating death domain protein MADD DEEAH, DENN, IG20, NEDDISH, RAB3GEP MAP kinase activating death domain MAP kinase-activating death domain protein|Rab3 GDP/GTP exchange factor|differentially expressed in normal and neoplastic cells|insulinoma glucagonoma clone 20
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MADD, check out the MADD Infographic
![MADD infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MADD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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