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Product Info Summary
| SKU: | EKC2241 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Keratin 18 (Phospho-Ser52) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2241
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Keratin 18 (Phospho-Ser52) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Keratin 18 (Phospho-Ser52) protein expression profile in cells. The kit can be used for measuring the relative amounts of Keratin 18 (Phospho-Ser52) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Keratin 18 (Phospho-Ser52).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Keratin 18 (Phospho-Ser52) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2241)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2241 is reactive to KRT18 in Human samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2241 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Keratin 18 Phospho-Ser52 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Keratin 18 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HepG2 cells
Protein Target Info & Infographic
Gene/Protein Information For KRT18 (Source: Uniprot.Org, NCBI)
Gene Name
KRT18
Full Name
Keratin, type I cytoskeletal 18
Weight
48058 MW
Superfamily
intermediate filament family
Alternative Names
CK 18;CK18;CYK18;Cytokeratin endo B;K18;K1C18;KERD;KRT1-18;KRT18;Keratin;Keratin D;Keratin;type I cytoskeletal 18;cytokeratin 18;type I cytoskeletal 18 KRT18 CK-18, CYK18, K18 keratin 18 keratin, type I cytoskeletal 18|cell proliferation-inducing gene 46 protein|cytokeratin 18|keratin 18, type I
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KRT18, check out the KRT18 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KRT18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Keratin 18 (Phospho-Ser52) Colorimetric Cell-Based ELISA Kit (EKC2241)
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