IL-1RA-Horse Interleukin-1 Receptor Antagonist Horse Recombinant Protein

Product Name

IL-1RA-Horse Interleukin-1 Receptor Antagonist Horse Recombinant Protein

View all IL-1ra/IL-1F3/IL1RN recombinant proteins

SKU/Catalog Number

PROTO18999

Size

20ug, 100ug, 1mg

Description

Recombinant Horse Interleukin-1 Receptor Antagonist produced in E.coli cells is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17.4kDa. The IL-1RA is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized IL-1RA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-1RA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

IL-1RA-Horse Interleukin-1 Receptor Antagonist Horse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTO18999)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The IL-1RA was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

20.055kDa

Reconstitution

It is recommended to reconstitute the lyophilized IL-1RA in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10.G4.1 helper T cells is less than 3.0 μg/ml, corresponding to a specific activity of greater than 333 IU/mg in the presence of 50 pg/ml rHuIL-1α.

Reconstitution

It is recommended to reconstitute the lyophilized IL-1RA in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For IL1RN (Source: Uniprot.org, NCBI)

Alternative Names

Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; IL1 inhibitor; IL1RN; IL1RA IL1RN DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4 interleukin 1 receptor antagonist interleukin-1 receptor antagonist protein|IL1 inhibitor|intracellular IL-1 receptor antagonist type II|intracellular interleukin-1 receptor antagonist (icIL-1ra)|type II interleukin-1 receptor antagonist

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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