IHH Indian Hedgehog Human Recombinant Protein

Product Name

IHH Indian Hedgehog Human Recombinant Protein

View all Indian Hedgehog/Ihh recombinant proteins

SKU/Catalog Number

PROTQ14623

Size

5ug, 25ug, 1mg

Description

IHH Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8kDa. The IHH is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized IHH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IHH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

IHH Indian Hedgehog Human Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ14623)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

45.251kDa

Reconstitution

It is recommended to reconstitute the lyophilized IHH in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED₅₀ for this effect is 3.0-10.0?g/ml corresponding to a specific activity of 100-334units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized IHH in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For IHH (Source: Uniprot.org, NCBI)

Alternative Names

Indian hedgehog protein; IHH; HHG-2; BDA1 IHH BDA1, HHG2 Indian hedgehog signaling molecule indian hedgehog protein|Indian hedgehog homolog

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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