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Product Info Summary
| SKU: | FEK1121 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit
See all BAFFR/TNFRSF13C products
SKU/Catalog Number
FEK1121
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFRSF13C/BAFFR PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TNFRSF13C/BAFFR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1121)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S7-A71
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1121 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1121 is reactive to Tnfrsf13c in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1121 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BAFFR/TNFRSF13C
Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1121-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1121-ST | Human BAFFR/TNFRSF13C Standard | 2 | 10 ng/tube |
| FEK1121-A | Human Tnfrsf13c Antibody A | 1 | 3ml |
| FEK1121-B | Human Tnfrsf13c Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
| O.D. | 0.031 | 0.120 | 0.192 | 0.341 | 0.604 | 1.071 | 1.875 | 2.426 |
Data Example Images
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Human TNFRSF13C PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 179 | 685 | 2752 | 158 | 702 | 2533 |
| Standard deviation | 13.06 | 51.37 | 217.40 | 13.43 | 61.07 | 210.23 |
| CV(%) | 7.3% | 7.5% | 7.9% | 8.5% | 8.7% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BAFFR/TNFRSF13C in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 179 | 164 | 137 | 140 | 155 | 10.54 | 6.8% |
| Sample 2 | 685 | 749 | 711 | 654 | 699 | 35.64 | 5.1% |
| Sample 3 | 2752 | 3118 | 2754 | 2810 | 2858 | 157.19 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf13c (Source: Uniprot.Org, NCBI)
Gene Name
Tnfrsf13c
Full Name
Tumor necrosis factor receptor superfamily member 13C
Weight
Alternative Names
BAFF R; BAFFR; BR3; CD268; TNFRSF13C Tnfrsf13c|2010006P15Rik, BAFF, BAFF-R, Baf, Baffr, Bcmd, Bcmd-1, Bcmd1, Lv, Lvis22|tumor necrosis factor receptor superfamily, member 13c|tumor necrosis factor receptor superfamily member 13C|B-cell maturation defect 1|B-cell-activating factor receptor|BAFF receptor|BLyS receptor 3|b cell-activating factor receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tnfrsf13c, check out the Tnfrsf13c Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfrsf13c: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit (FEK1121)
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