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Product Info Summary
| SKU: | EK0917 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human Thrombomodulin ELISA Kit PicoKine®
View all Thrombomodulin/BDCA-3 ELISA kits
SKU/Catalog Number
EK0917
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Thrombomodulin ELISA Kit PicoKine® (96 Tests). Quantitate Human THBD in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Thrombomodulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0917)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A19-S515
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0917 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0917 is reactive to THBD in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
EK0917 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Thrombomodulin/BDCA-3
Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene (THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome (aHUS).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0917-CAP | Anti-Human THBD Pre-coated 96-well strip microplate | 1 |
| EK0917-ST | Human THBD Standard | 2 vials, 10 ng/tube |
| EK0917-DA | Human THBD Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.001 | 0.065 | 0.123 | 0.224 | 0.421 | 0.801 | 1.387 | 2.010 |
Data Example Images
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Human Thrombomodulin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Thrombomodulin/BDCA-3, Dilution ratio of 1:5, concentration in serum and plasma is 2292-6381 pg/ml..
Some articles we found to cite concentrations of Thrombomodulin/BDCA-3 in samples: 16405768 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Thrombomodulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 164 | 418 | 1319 | 176 | 453 | 1243 |
| Standard deviation | 7.38 | 26.33 | 79.14 | 9.68 | 33.52 | 91.98 |
| CV (%) | 4.5% | 6.3% | 6% | 5.5% | 7.4% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Thrombomodulin/BDCA-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 164 | 165 | 162 | 171 | 165 | 3.35 | 2% |
| Sample 2 | 418 | 416 | 394 | 403 | 407 | 9.8 | 2.4% |
| Sample 3 | 1319 | 1304 | 1439 | 1459 | 1380 | 69.31 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For THBD (Source: Uniprot.Org, NCBI)
Gene Name
THBD
Full Name
Thrombomodulin
Weight
60329 MW
Alternative Names
Thrombomodulin;TM;Fetomodulin;CD141;THBD;THRM; THBD AHUS6, BDCA-3, BDCA3, CD141, THPH12, THRM, TM thrombomodulin thrombomodulin|CD141 |fetomodulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on THBD, check out the THBD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for THBD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Thrombomodulin ELISA Kit PicoKine® (EK0917)
Hello CJ!
EK0917 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The effects of moderate and high-intensity exercise on circulating markers of endothelial integrity and activation in young, healthy men
miR‐499 released during myocardial infarction causes endothelial injury by targeting α7‐nAchR
Blood Hemostatic Changes During an Ultraendurance Road Cycling Event in a Hot Environment
Kupchak, B.R., Kazman, J.B., Vingren, J.L., Levitt, D.E., Lee, E.C., Williamson, K.H.,..., & Deuster, P.A. (2017). Blood Hemostatic Changes During an Ultraendurance Road Cycling Event in a Hot Environment. Wilderness & Environmental Medicine. Adva...
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human Thrombomodulin ELISA Kit PicoKine®
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
G. King
Verified customer
Asked: 2020-09-17
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-09-17
Question
Q: how to analyze ELISA data? I measured Thrombomodulin level in urine.
W. Wilson
Verified customer
Asked: 2020-09-04
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-09-04
Question
Q: can I use citrate plasma as samples in Human Thrombomodulin Picokine® ELISA Kit (Catalog # EK0917)?
Verified Customer
Verified customer
Asked: 2019-12-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Thrombomodulin causing degradation of its protein structure. Thrombomodulin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Thrombomodulin ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or Citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-12-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-19
Question
Q: Can Thrombomodulin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Walker
Verified customer
Asked: 2018-04-17
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-17
Question
What is the average level of Human Thrombomodulin reported?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
Thrombomodulin level in healthy human serum/plasma is about 2820-5398 pg/ml.
Boster Scientific Support
Answered: 2018-01-04
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Thrombomodulin in Human urine? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
T. Clark
Verified customer
Asked: 2017-03-04
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Thrombomodulin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-03-04
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
H. Mangal
Verified customer
Asked: 2015-06-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-06-27
Question
I did not get any measurable Thrombomodulin signal in my cell culture supernatants on ELISA but I expected some baseline signal even if it was small. Meanwhile, the standards worked fine, and I also confirmed cell activation by flow cytometry with ICAM-1. What happened? Any troubleshooting suggestions?
Verified Customer
Verified customer
Asked: 2014-09-04
Answer
Confirmed cell activation doesn't indicate the sample contains protein of interest. The protein may also be in small quantities beyond the ELISA detection range. To troubleshoot, we suggest that you try to increase the cell density and/or homogenize/lyse the cells in lysis buffer (instead of working with cell supernatant).
Boster Scientific Support
Answered: 2014-09-04
