Product Info Summary
SKU: | EK1171 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human SP-D / Surfactant protein D ELISA Kit PicoKine®
SKU/Catalog Number
EK1171
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SP-D / Surfactant protein D ELISA Kit PicoKine® (96 Tests). Quantitate Human SFTPD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SP-D / Surfactant protein D ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1171)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A21-F375
Sensitivity
<20 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1171 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1171 is reactive to SFTPD in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1171 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SP-D
Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1171-CAP | Anti-Human SFTPD Pre-coated 96-well strip microplate | 1 |
EK1171-ST | Human SFTPD Standard | 2 vials, 40 ng/tube |
EK1171-DA | Human SFTPD Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.049 | 0.188 | 0.306 | 0.514 | 0.979 | 1.383 | 1.708 | 2.056 |
Data Example Images
Click image to see more details
Human SP-D PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SP-D, Dilution ratio of 1:1, concentration in serum and plasma is around 2.6 ng/ml..
Some articles we found to cite concentrations of SP-D in samples: 25488319 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SP-D / Surfactant protein D ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1547 | 3580 | 25269 | 1591 | 3588 | 23717 |
Standard deviation | 92.82 | 221.96 | 1693.02 | 111.37 | 279.86 | 1683.9 |
CV (%) | 6% | 6.2% | 6.7% | 7% | 7.8% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SP-D in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 1547 | 1291 | 1390 | 1463 | 1422 | 94.19 | 6.6% |
Sample 2 | 3580 | 3482 | 3689 | 3752 | 3625 | 103.31 | 2.8% |
Sample 3 | 25269 | 29048 | 29153 | 26765 | 27558 | 1630.37 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For SFTPD (Source: Uniprot.Org, NCBI)
Gene Name
SFTPD
Full Name
Pulmonary surfactant-associated protein D
Weight
37728 MW
Superfamily
SFTPD family
Alternative Names
Pulmonary surfactant-associated protein D;PSP-D;SP-D;Collectin-7;Lung surfactant protein D;SFTPD;COLEC7, PSPD, SFTP4; SFTPD COLEC7, PSP-D, SFTP4, SP-D surfactant protein D pulmonary surfactant-associated protein D|collectin-7|lung surfactant protein D|pulmonary surfactant apoprotein|surfactant-associated protein, pulmonary 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SFTPD, check out the SFTPD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SFTPD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SP-D / Surfactant protein D ELISA Kit PicoKine® (EK1171)
Hello CJ!
EK1171 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum surfactant protein D in COVID-19 is elevated and correlated with disease severity
Surfactant Protein-D: A sensitive predictor for efficiency of preoperative pulmonary rehabilitation
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Human SP-D / Surfactant protein D ELISA Kit PicoKine®
Question
What is the assay range of EK1171?
Verified customer
Asked: 2021-02-05
Answer
The assay range of the Human SP-D / Surfactant Protein D ELISA Kit PicoKine™ (EK1171) is 0.625ng/ml-40ng/ml.
Boster Scientific Support
Answered: 2021-02-07
Question
What is the concentration range of SP-D in serum samples of EK1171?
Verified customer
Asked: 2021-02-05
Answer
The concentration range of SP-D in serum samples of the Human SP-D / Surfactant Protein D ELISA Kit PicoKine™ (EK1171) is 0.8-20ng/ml.
Boster Scientific Support
Answered: 2021-02-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
T. Mitchell
Verified customer
Asked: 2020-03-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-03-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Martinez
Verified customer
Asked: 2020-02-16
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-02-16
Question
What is the immunized animal and purification way of the antibody in EK1171?
Verified customer
Asked: 2019-10-02
Answer
The capture antibody of the Human SP-D / Surfactant Protein D ELISA Kit PicoKine® (EK1171) is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat. The purification method of the antibody used is affinity purified.
Boster Scientific Support
Answered: 2019-10-08
Question
Q: Can SP-D ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-04-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
R. Hall
Verified customer
Asked: 2018-08-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-21
Question
Q: how do I analyze ELISA data? I measured SP-D level in cell lysates.
C. Bhatt
Verified customer
Asked: 2018-04-03
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-04-03
Question
Q: if the enzyme conjugated SP-D antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SP-D antigen?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-27
Question
Q: how to thaw whole blood sample for SP-D ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-06-13
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SP-D for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-06-13