Human recombinant IL-30 (Interleukin-30) protein, AF

IL27 protein, Human

Interleukin 30 (IL-30) is a 27 kDa cytokine with 216 amino acid residues. IL-30, also known as IL-27A, forms a complex named IL-27 with Ebi3 and is secreted from cells of myeloid origin, such as monocytes, macrophages, and dendritic cells. IL-30 /Ebi3 complex mediates T-cell immunity, promoting Th1 differentiation, inhibiting Th2 differentiation, and inhibiting Th17 differentiation. IL-30 also can be secreted independently of Ebi3 and modulate bacteria-induced oxidative burst and cytokine production.

Product Info Summary

SKU: PROTQ8NEV9-1
Size: 5ug,20ug,100ug
Origin Species: Human
Source: Escherichia coli
Application: Cell Culture

Product Name

Human recombinant IL-30 (Interleukin-30) protein, AF

View all IL27 recombinant proteins

SKU/Catalog Number

PROTQ8NEV9-1

Size

5ug,20ug,100ug

Tag

His Tag (C-term)

Description

Interleukin 30 (IL-30) is a 27 kDa cytokine with 216 amino acid residues. IL-30, also known as IL-27A, forms a complex named IL-27 with Ebi3 and is secreted from cells of myeloid origin, such as monocytes, macrophages, and dendritic cells. IL-30 /Ebi3 complex mediates T-cell immunity, promoting Th1 differentiation, inhibiting Th2 differentiation, and inhibiting Th17 differentiation. IL-30 also can be secreted independently of Ebi3 and modulate bacteria-induced oxidative burst and cytokine production.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Human recombinant IL-30 (Interleukin-30) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ8NEV9-1)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.

Purity

>98% as determined by SDS-PAGE.

Predicted MW

27.493kDa

Molecular weight

The protein has a calculated MW of 25.47 kDa. The protein migrates as 28 kDa under reducing condition (SDS-PAGE analysis).

Activity

Testing in process

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

MFPRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPGVNLYLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGTQGRWTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEEERKGLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLSKAGHSVWPLGFPTLSPQP with polyhistidine tag at the C-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For IL27 (Source: Uniprot.org, NCBI)

Gene Name

IL27

Full Name

Interleukin-27 subunit alpha

Weight

27.493kDa

Superfamily

IL-6 superfamily

Alternative Names

IL-27-p28 subunit, IL27A; IL-27A; IL27p28 IL27 IL-27, IL-27AA, IL27p28, IL30, p28, IL27 interleukin 27 interleukin-27 subunit alpha|IL-27 p28 subunit|IL-27 subunit alpha|IL-27-A|IL27-A|interleukin-30

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IL27, check out the IL27 Infographic

IL27 infographic

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In this infographic, you will see the following information for IL27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTQ8NEV9-1

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