Human recombinant IFN omega (interferon omega) protein, AF

IFNW1 protein, Human

Interferon Omega (IFN-ω) is a 20.12 kDa member of type I IFN family with 173 amino acid residues. IL-28B is expressed by epithelial tissues. IFN-ω with antiviral, antitumor activity and regulating the innate immune response. Able to activate P13K/Akt signaling pathway via binding its receptor IFNAR in cells.

Product Info Summary

SKU: PROTP05000-3
Size: 20ug,100ug,500ug,1mg
Origin Species: Human
Source: Escherichia coli
Application: Cell Culture

Product Name

Human recombinant IFN omega (interferon omega) protein, AF

View all IFNW1 recombinant proteins

SKU/Catalog Number

PROTP05000-3

Size

20ug,100ug,500ug,1mg

Tag

His Tag (C-term)

Description

Interferon Omega (IFN-ω) is a 20.12 kDa member of type I IFN family with 173 amino acid residues. IL-28B is expressed by epithelial tissues. IFN-ω with antiviral, antitumor activity and regulating the innate immune response. Able to activate P13K/Akt signaling pathway via binding its receptor IFNAR in cells.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Human recombinant IFN omega (interferon omega) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP05000-3)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Purity

>95% as determined by SDS-PAGE.

Predicted MW

22.319kDa

Molecular weight

The protein has a calculated MW of 20.93 kDa. The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).

Activity

Measure by its ability to induce cytotoxicity in TF-1 cells. The ED₅₀ for this effect is <0.02 ng/mL. The specific activity of recombinant human IFN omega is approximately >5 x10⁷ IU/ mg.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

MCDLPQNHGLLSRNTLVLLHQMRRISPFLCLKDRRDFRFPQEMVKGSQLQKAHVMSVLHEMLQQIFSLFHTERSSAAWNMTLLDQLHTGLHQQLQHLETCLLQVVGEGESAGAISSPALTLRRYFQGIRVYLKEKKYSDCAWEVVRMEIMKSLFLSTNMQERLRSKDRDLGSS with polyhistidine tag at the C-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For IFNW1 (Source: Uniprot.org, NCBI)

Gene Name

IFNW1

Full Name

Interferon omega-1

Weight

22.319kDa

Superfamily

alpha/beta interferon family

Alternative Names

IFN alpha II-1, IFNW1 IFNW1 interferon omega 1 interferon omega-1|IFN-omega 1, interferon omega-1|interferon alpha-II-1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IFNW1, check out the IFNW1 Infographic

IFNW1 infographic

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In this infographic, you will see the following information for IFNW1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTP05000-3

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Lead time for this item is typically 2-4 weeks

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