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Product Info Summary
| SKU: | PROTP51858-2 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant HDGF (Hepatoma-derived growth factor) protein, AF
View all HDGF recombinant proteins
SKU/Catalog Number
PROTP51858-2
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Heparin binding Growth Factors (HDGF) is a 27.60 kDa hepatoma-derived Growth Factors with 246 amino acid residues. HDGF is expressed from mitochondria and proteasome. Functionally, it is a potent mitogen, stimulating the growth of vascular smooth muscle cells, hepatoma cells and endothelial cells, acting as a transcriptional repressor.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant HDGF (Hepatoma-derived growth factor) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP51858-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
26.788kDa
Molecular weight
The protein has a calculated MW of 27.60 kDa. The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human HDGF
Protein Target Info & Infographic
Gene/Protein Information For HDGF (Source: Uniprot.org, NCBI)
Gene Name
HDGF
Full Name
Hepatoma-derived growth factor
Weight
26.788kDa
Superfamily
HDGF family
Alternative Names
HMG1L2 HDGF HMG1L2 heparin binding growth factor hepatoma-derived growth factor|epididymis secretory sperm binding protein|hepatoma derived growth factor|high mobility group protein 1-like 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on HDGF, check out the HDGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant HDGF (Hepatoma-derived growth factor) protein, AF (PROTP51858-2)
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