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Product Info Summary
| SKU: | PROTP06733-2 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant ENO1 protein, AF
View all Enolase 1 recombinant proteins
SKU/Catalog Number
PROTP06733-2
Size
5ug,20ug,100ug
Tag
His Tag (N-term)
Description
Enolase 1 (ENO1) also named alpha-enolase which is a glycolytic enzyme. It could convert the NAD+ to NADH by producing two ATP. ENO1 is a homodimer which is composed by two isozymes of enolase (2 α, 2 ν, or 2 β). It also plays an important role in cancer model which can promote tumor cell proliferation and migration by the PI3K signaling pathway. ENO1 is a biomarker of prognostic and diagnostic cancer.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant ENO1 protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP06733-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 7 mM MgSO4 in 1X PBS, pH 7.2 If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
47.169kDa
Molecular weight
The protein has a calculated MW of 47.85 kDa. The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
SILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK with polyhistidine tag at the N-terminus
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human ENO1
Protein Target Info & Infographic
Gene/Protein Information For ENO1 (Source: Uniprot.org, NCBI)
Gene Name
ENO1
Full Name
Alpha-enolase
Weight
47.169kDa
Superfamily
enolase family
Alternative Names
enolase 1, NNE, PPH, MPB1, ENO1L1, HEL-S-17 ENO1 ENO1L1, HEL-S-17, MPB1, NNE, PPH enolase 1 alpha-enolase|c-myc promoter-binding protein-1|2-phospho-D-glycerate hydro-lyase|MYC promoter-binding protein 1|alpha enolase like 1|enolase 1, (alpha)|enolase-alpha|epididymis secretory protein Li 17|non-neural enolase|phosphopyruvate hydratase|plasminogen-binding protein|tau-crystallin
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ENO1, check out the ENO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant ENO1 protein, AF (PROTP06733-2)
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