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Product Info Summary
| SKU: | M01250-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Equine, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Enolase 1 ENO1 Monoclonal Antibody
SKU/Catalog Number
M01250-1
Size
100ul
Description
Boster Bio Anti-Enolase 1 ENO1 Monoclonal Antibody catalog # M01250-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Enolase 1 ENO1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01250-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
N-terminal 12 amino acids of bovine enolase 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01250-1 is reactive to ENO1 in Bovine, Equine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
47169 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01250-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000-1:10,000 IF/ICC and IHC: 1:2,000-1:5,000.
Validation Images & Assay Conditions
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Western blot analysis of different cell lysates using mouse mAb to -enolase, M01250-1, dilution 1:10,000 in green: [1] protein standard (red), [2] NIH-3T3 l, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cells. A strong single band at 47kDa corresponds to the -enolase protein.
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Immunofluorescent analysis of HeLa cells stained with mouse mAb to -enolase, M01250-1, dilution 1:500 in green and costained with chicken pAb to HSP60, dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. The M01250-1 antibody reveals strong cytoplasmic staining while the other antibody specifically labels mitochondria in these cells.
Protein Target Info & Infographic
Gene/Protein Information For ENO1 (Source: Uniprot.org, NCBI)
Gene Name
ENO1
Full Name
Alpha-enolase
Weight
47169 MW
Superfamily
enolase family
Alternative Names
Alpha-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;C-myc promoter-binding protein;Enolase 1;MBP-1;MPB-1;Non-neural enolase;NNE;Phosphopyruvate hydratase;Plasminogen-binding protein;ENO1;ENO1L1, MBPB1, MPB1; ENO1 ENO1L1, HEL-S-17, MPB1, NNE, PPH enolase 1 alpha-enolase|c-myc promoter-binding protein-1|2-phospho-D-glycerate hydro-lyase|MYC promoter-binding protein 1|alpha enolase like 1|enolase 1, (alpha)|enolase-alpha|epididymis secretory protein Li 17|non-neural enolase|phosphopyruvate hydratase|plasminogen-binding protein|tau-crystallin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENO1, check out the ENO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Enolase 1 ENO1 Monoclonal Antibody (M01250-1)
Hello CJ!
M01250-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Y,Li L,Qiu M,Tan L,Zhang M,Li J,Zhu H,Jiang S,Su X,Li A.Renal and cerebral RAS interaction contributes to diabetic kidney disease.Am J Transl Res.2019 May 15;11(5):2925-2939.PMID:31217864;PMCID:PMC6556645.
Species: Rat
M01250-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:NA
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Customer Q&As
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1 Customer Q&As for Anti-Enolase 1 ENO1 Monoclonal Antibody
Question
We are currently using anti-Enolase 1 Monoclonal antibody M01250-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The anti-Enolase 1 Monoclonal antibody (M01250-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-30
