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Product Info Summary
| SKU: | PROTP10747-2 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | HEK293 cell |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant CD28 ECD protein, AF
View all CD28 recombinant proteins
SKU/Catalog Number
PROTP10747-2
Size
5ug,20ug,100ug
Tag
His-SUMO Tag (N-term)
Description
CD28, a well-known costimulatory receptor expressed on T cells, is a type I transmembrane glycoprotein of the Ig superfamily. When the T cells receive the first signal delivered by APCs (antigen-presenting cells), it initiates signaling cascades, which favor T cell survival and proliferation. It has been demonstrated that patients with lung cancer who responded to PD-1 therapy had more CD28+ T cells, suggesting that CD28 may predict treatment response.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant CD28 ECD protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP10747-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
25.066kDa
Molecular weight
The protein has a calculated MW of 28.61 kDa. The protein migrates as 48-62 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
A DNA sequence encoding Human CD28 ECD (# P10747) (Asn19 – Pro152) was expressed with polyhistidine-SUMO tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human CD28 ECD
Protein Target Info & Infographic
Gene/Protein Information For CD28 (Source: Uniprot.org, NCBI)
Gene Name
CD28
Full Name
T-cell-specific surface glycoprotein CD28
Weight
25.066kDa
Alternative Names
TP44 CD28 Tp44 CD28 molecule T-cell-specific surface glycoprotein CD28
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CD28, check out the CD28 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant CD28 ECD protein, AF (PROTP10747-2)
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