Human recombinant BMP-3 (Bone morphogenetic protein-3) protein, AF

BMP-3 protein, Human

Bone Morphogenetic Protein-3 (BMP-3) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-3 can bind with TGF-β receptor and participate in SMAD protein signal transduction through triggering pathway-restricted SMAD protein phosphorylation. The primary function of BMP-3 is osteoblast differentiation and Induces bone formation.

Product Info Summary

SKU: PROTP12645-2
Size: 5ug,20ug,100ug
Origin Species: Human
Source: Escherichia coli
Application: Cell Culture

Product Name

Human recombinant BMP-3 (Bone morphogenetic protein-3) protein, AF

View all BMP-3 recombinant proteins

SKU/Catalog Number

PROTP12645-2

Size

5ug,20ug,100ug

Tag

His Tag (C-term)

Description

Bone Morphogenetic Protein-3 (BMP-3) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-3 can bind with TGF-β receptor and participate in SMAD protein signal transduction through triggering pathway-restricted SMAD protein phosphorylation. The primary function of BMP-3 is osteoblast differentiation and Induces bone formation.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Human recombinant BMP-3 (Bone morphogenetic protein-3) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP12645-2)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.

Purity

>95% as determined by SDS-PAGE.

Predicted MW

53.372kDa

Molecular weight

The protein has a calculated MW of 13.34 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).

Activity

Measure by its ability to inhibit BMP-2-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is < 10 μg/mL.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

MQWIEPRNCARRYLKVDFADIGWSEWIISPKSFDAYYCSGACQFPMPKSLKPSNHATIQSIVRAVGVVPGIPEPCCVPEKMSSLSILFFDENKNVVLKVYPNMTVESCACR with polyhistidine tag at the C-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For BMP3 (Source: Uniprot.org, NCBI)

Gene Name

BMP3

Full Name

Bone morphogenetic protein 3

Weight

53.372kDa

Superfamily

TGF-beta family

Alternative Names

Osteogenin, BMP-3A BMP3 BMP-3A bone morphogenetic protein 3 bone morphogenetic protein 3|bone morphogenetic protein 3 (osteogenic)|bone morphogenetic protein 3A|osteogenin

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on BMP3, check out the BMP3 Infographic

BMP3 infographic

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In this infographic, you will see the following information for BMP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTP12645-2

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