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Product Info Summary
| SKU: | EK2261 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human PIGR ELISA Kit PicoKine®
SKU/Catalog Number
EK2261
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PIGR ELISA Kit PicoKine® (96 Tests). Quantitate Human PIGR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PIGR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2261)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: K19-R638
Sensitivity
<150 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2261 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2261 is reactive to PIGR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2261 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PIGR
Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2261-CAP | Anti-Human PIGR Pre-coated 96-well strip microplate | 1 |
| EK2261-ST | Human PIGR Standard | 2 vials, 20 ng/tube |
| EK2261-DA | Human PIGR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.030 | 0.065 | 0.104 | 0.273 | 0.472 | 0.929 | 1.465 | 2.069 |
Data Example Images
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Human PIGR PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PIGR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 557 | 804 | 6554 | 537 | 1230 | 6237 |
| Standard deviation | 27.85 | 37.87 | 250.36 | 41.56 | 57.93 | 343.66 |
| CV(%) | 5% | 4.7% | 3.8% | 7.7% | 4.7% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PIGR in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 557 | 545 | 490 | 463 | 514 | 31.7 | 6.2% |
| Sample 2 | 804 | 1174 | 792 | 635 | 851 | 37.46 | 4.4% |
| Sample 3 | 6554 | 7594 | 7529 | 7820 | 7374 | 447.62 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For PIGR (Source: Uniprot.Org, NCBI)
Gene Name
PIGR
Full Name
Polymeric immunoglobulin receptor
Weight
Alternative Names
FLJ22667; Free Secretory Component (FSC) ; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgA-Secretory Component Complex; pIgM-Secretory Component Complex; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor; Secretory Component (SC); sIgA-Secretory Component Complex PIGR polymeric immunoglobulin receptor polymeric immunoglobulin receptor|hepatocellular carcinoma associated protein TB6|poly-Ig receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PIGR, check out the PIGR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PIGR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PIGR ELISA Kit PicoKine® (EK2261)
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