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Product Info Summary
| SKU: | EK2272 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine®
View all Olfactomedin-2/Noelin-2 ELISA kits
SKU/Catalog Number
EK2272
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human Olfactomedin-2/Noelin-2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 25 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2272)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: Q21-P454
Sensitivity
<25 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2272 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2272 is reactive to OLFM2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2272 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Olfactomedin-2/Noelin-2
Olfactomedin 2, also known as noelin 2, is a protein that in humans is encoded by the OLFM2 gene. OLFM2 is an olfactomedin domain-containing protein that is involved in early eye development and function. It also plays a role in TGFB-mediated differentiation of smooth muscle cells (SMCs) from mesenchymal stem cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2272-CAP | Anti-Human OLFM2 Pre-coated 96-well strip microplate | 1 |
| EK2272-ST | Human OLFM2 Standard | 2 vials, 10 ng/tube |
| EK2272-DA | Human OLFM2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1,500 | 3,000 |
| O.D. | 0.023 | 0.039 | 0.116 | 0.262 | 0.439 | 0.876 | 1.341 | 1.867 |
Data Example Images
Click image to see more details
Human Olfactomedin-2/Noelin-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 49 | 139 | 970 | 59 | 123 | 838 |
| Standard deviation | 1.86 | 6.27 | 46.66 | 2.94 | 7.49 | 31.26 |
| CV(%) | 3.8% | 4.5% | 4.8% | 5% | 6.1% | 3.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Olfactomedin-2/Noelin-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 49 | 62 | 49 | 64 | 56 | 1.25 | 2.2% |
| Sample 2 | 139 | 107 | 110 | 187 | 136 | 4.91 | 3.6% |
| Sample 3 | 970 | 911 | 1371 | 1205 | 1114 | 71.87 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For OLFM2 (Source: Uniprot.Org, NCBI)
Gene Name
OLFM2
Full Name
Noelin-2
Weight
Alternative Names
neuronal olfactomedin related ER localized protein 2; NOE2; NOE2noelin 2; NOELIN2; Noelin-2; NOELIN2_V1; olfactomedin 2; Olfactomedin2; Olfactomedin-2; OlfC; OLFM2; OM2 OLFM2 NOE2, NOELIN2, NOELIN2_V1, OlfC olfactomedin 2 noelin-2|neuronal olfactomedin related ER localized protein 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on OLFM2, check out the OLFM2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for OLFM2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine® (EK2272)
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