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Product Info Summary
| SKU: | EK2250 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human NCR2 ELISA Kit PicoKine®
View all NKp44/NCR2 ELISA kits
SKU/Catalog Number
EK2250
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human NCR2 PicoKine ELISA Kit® (96 Tests). Quantitate Human NCR2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 25 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human NCR2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2250)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: Q22-P190
Sensitivity
<25 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2250 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2250 is reactive to NCR2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2250 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NKp44/NCR2
Natural cytotoxicity triggering receptor 2 is a protein that in humans is encoded by the NCR2 gene. Predicted to enable signaling receptor activity. Predicted to be involved in cellular defense response and signal transduction. Predicted to be located in plasma membrane. Predicted to be integral component of plasma membrane. Predicted to be active in cell surface.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2250-CAP | Anti-Human NCR2 Pre-coated 96-well strip microplate | 1 |
| EK2250-ST | Human NCR2 Standard | 2 vials, 10 ng/tube |
| EK2250-DA | Human NCR2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.013 | 0.032 | 0.055 | 0.169 | 0.418 | 0.849 | 1.310 | 1.830 |
Data Example Images
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Human NCR2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human NCR2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 82 | 109 | 1149 | 72 | 113 | 824 |
| Standard deviation | 2.76 | 8.57 | 86.06 | 1.82 | 2.94 | 22.82 |
| CV(%) | 3.4% | 7.9% | 7.5% | 2.5% | 2.6% | 2.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of NKp44/NCR2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 82 | 89 | 65 | 69 | 76 | 2.14 | 2.8% |
| Sample 2 | 109 | 112 | 173 | 168 | 141 | 4 | 2.9% |
| Sample 3 | 1149 | 1259 | 1052 | 1227 | 1172 | 77.57 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For NCR2 (Source: Uniprot.Org, NCBI)
Gene Name
NCR2
Full Name
Natural cytotoxicity triggering receptor 2
Weight
Superfamily
natural cytotoxicity receptor (NCR) family
Alternative Names
CD336 antigen; CD336; LY95; LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44); lymphocyte antigen 95 (activating NK-receptor; NK-p44); Lymphocyte antigen 95 homolog; natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; NCR2; NK cell activating receptor (NKp44); NK cell-activating receptor; NKp44; NK-p44dJ149M18.1 NCR2 CD336, LY95, NK-p44, NKP44, dJ149M18.1 natural cytotoxicity triggering receptor 2 natural cytotoxicity triggering receptor 2|NK cell activating receptor (NKp44)|NK cell-activating receptor|lymphocyte antigen 95 (activating NK-receptor; NK-p44)|lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44)|natural killer cell p44-related protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NCR2, check out the NCR2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NCR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human NCR2 ELISA Kit PicoKine® (EK2250)
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