Human Napsin A ELISA Kit PicoKine®

Napsin A ELISA kit for Human

Human Napsin A PicoKine® ELISA Kit (96 Tests). Quantitate Human Napsin A in cell culture supernatants, serum and plasma (heparin). Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2249
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)

Product Name

Human Napsin A ELISA Kit PicoKine®

View all Napsin A ELISA kits

SKU/Catalog Number

EK2249

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Napsin A PicoKine® ELISA Kit (96 Tests). Quantitate Human Napsin A in cell culture supernatants, serum and plasma (heparin). Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Napsin A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2249)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: CHO; Immunogen sequence: T25-G420

Sensitivity

<30 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2249 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2249 is reactive to NAPSA in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

EK2249 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Napsin A

Napsin-A is an aspartic proteinase that is encoded in humans by the NAPSA gene. This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma.

Kit Components

Catalog Number Description Quantity
EK2249-CAP Anti-Human NAPSA Pre-coated 96-well strip microplate 1
EK2249-ST Human NAPSA Standard 2 vials, 10 ng/tube
EK2249-DA Human NAPSA Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)062.51252505001,0002,0004,000
O.D.0.0390.0680.1890.2980.5210.8851.2141.912

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Napsin A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)1061541205822371482
Standard deviation3.933.2650.493.6218.9440.61
CV(%)3.7%2.1%4.2%4.4%8%2.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Napsin A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11067710678923.94.3%
Sample 21542091822471987.783.9%
Sample 31205197911521246139666.154.7%
*number of samples for each test n=16.

Gene/Protein Information For NAPSA (Source: Uniprot.Org, NCBI)

Gene Name

NAPSA

Full Name

Napsin-A

Weight

45.387kDa

Superfamily

peptidase A1 family

Alternative Names

Asp 4; ASP4; Aspartyl protease 4; EC 3.4.23; EC 3.4.23.-; EC 3.4.23.15; EC 3.4.23.3; EC 3.4.23.5; KAP; Kdap; NAP1napsin-A; NAPA; NAPApronapsin A; NAPSA; napsin A aspartic peptidase; Napsin A; Napsin-1; SNAPA; TA01/TA02 NAPSA KAP, Kdap, NAP1, NAPA, SNAPA napsin A aspartic peptidase napsin-A|ASP4|TA01/TA02|asp 4|aspartyl protease 4|kidney-derived aspartic protease-like protein|napsin-1|pronapsin A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NAPSA, check out the NAPSA Infographic

NAPSA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NAPSA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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