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Product Info Summary
| SKU: | EK2249 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin) |
Product info
Product Name
Human Napsin A ELISA Kit PicoKine®
SKU/Catalog Number
EK2249
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Napsin A PicoKine® ELISA Kit (96 Tests). Quantitate Human Napsin A in cell culture supernatants, serum and plasma (heparin). Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Napsin A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2249)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: T25-G420
Sensitivity
<30 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2249 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2249 is reactive to NAPSA in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
Application Guarantee
EK2249 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Napsin A
Napsin-A is an aspartic proteinase that is encoded in humans by the NAPSA gene. This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2249-CAP | Anti-Human NAPSA Pre-coated 96-well strip microplate | 1 |
| EK2249-ST | Human NAPSA Standard | 2 vials, 10 ng/tube |
| EK2249-DA | Human NAPSA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.039 | 0.068 | 0.189 | 0.298 | 0.521 | 0.885 | 1.214 | 1.912 |
Data Example Images
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Human Napsin A PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Napsin A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 106 | 154 | 1205 | 82 | 237 | 1482 |
| Standard deviation | 3.93 | 3.26 | 50.49 | 3.62 | 18.94 | 40.61 |
| CV(%) | 3.7% | 2.1% | 4.2% | 4.4% | 8% | 2.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Napsin A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 106 | 77 | 106 | 78 | 92 | 3.9 | 4.3% |
| Sample 2 | 154 | 209 | 182 | 247 | 198 | 7.78 | 3.9% |
| Sample 3 | 1205 | 1979 | 1152 | 1246 | 1396 | 66.15 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For NAPSA (Source: Uniprot.Org, NCBI)
Gene Name
NAPSA
Full Name
Napsin-A
Weight
45.387kDa
Superfamily
peptidase A1 family
Alternative Names
Asp 4; ASP4; Aspartyl protease 4; EC 3.4.23; EC 3.4.23.-; EC 3.4.23.15; EC 3.4.23.3; EC 3.4.23.5; KAP; Kdap; NAP1napsin-A; NAPA; NAPApronapsin A; NAPSA; napsin A aspartic peptidase; Napsin A; Napsin-1; SNAPA; TA01/TA02 NAPSA KAP, Kdap, NAP1, NAPA, SNAPA napsin A aspartic peptidase napsin-A|ASP4|TA01/TA02|asp 4|aspartyl protease 4|kidney-derived aspartic protease-like protein|napsin-1|pronapsin A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NAPSA, check out the NAPSA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NAPSA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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