Human/Mouse COX-2 ELISA Kit PicoKine®

Product Name

Human/Mouse COX-2 ELISA Kit PicoKine®

View all COX-2 ELISA kits

SKU/Catalog Number

EK2289

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human/Mouse COX-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human/Mouse COX-2 in cell lysates. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human/Mouse COX-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2289)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A18-L604

Sensitivity

<20 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of EK2289 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2289 is reactive to PTGS2 in Human, Mouse samples

Validated Sample Types

cell lysates.

Application Guarantee

EK2289 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of COX-2

Cyclooxygenase-2 (COX-2), also known as Prostaglandin-endoperoxide synthase 2 (HUGO PTGS2), is an enzyme that in humans is encoded by the PTGS2 gene. PTGS2 (prostaglandin-endoperoxide synthase 2) is a gene that encodes an enzyme responsible for catalyzing the conversion of arachidonic acid into prostaglandins. Prostaglandins are lipid signaling molecules that play crucial roles in regulating inflammation, pain, and reproductive processes. PTGS2 is expressed in various tissues, particularly in sites of inflammation and certain tumor cells, where its expression levels are often significantly elevated. The activity of this gene is regulated by various stimuli, including cytokines, hormones, and growth factors, making it a key player in pathological conditions such as inflammation, cancer, and cardiovascular diseases. Due to its involvement in these diseases, PTGS2 has become a potential drug target, especially in the development of nonsteroidal anti-inflammatory drugs (NSAIDs).

Kit Components

Catalog Number	Description	Quantity
EK2289-CAP	Anti-Human, Mouse PTGS2 Pre-coated 96-well strip microplate	1
EK2289-ST	Human, Mouse PTGS2 Standard	2 vials, 10 ng/tube
EK2289-DA	Human, Mouse PTGS2 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.024	0.069	0.196	0.479	0.657	0.941	1.274	1.814

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human/Mouse COX-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of COX-2 in ELISA kits from four different production batches/lots.

Gene/Protein Information For PTGS2 (Source: Uniprot.Org, NCBI)

Alternative Names

COX2; COX-2; COX2cyclooxygenase 2b; cyclooxygenase-2; EC 1.14.99; EC 1.14.99.1; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; PHS-II; prostaglandin G/H synthase 2; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2 PTGS2 COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2 prostaglandin-endoperoxide synthase 2 prostaglandin G/H synthase 2|PGH synthase 2|PHS II|cyclooxygenase 2|cyclooxygenase 2b|prostaglandin H2 synthase 2|prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis