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Product Info Summary
| SKU: | EK0458 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human MMP-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0458
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human MMP1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0458)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F20-N469
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0458 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0458 is reactive to MMP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0458 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-1
Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0458-CAP | Anti-Human MMP1 Pre-coated 96-well strip microplate | 1 |
| EK0458-ST | Human MMP1 Standard | 2 vials, 10 ng/tube |
| EK0458-DA | Human MMP1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.000 | 0.077 | 0.130 | 0.258 | 0.514 | 0.964 | 1.574 | 2.091 |
Data Example Images
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Human MMP-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-1, For serum, dilution ratio is 1:1 and concentration is 0.72-11.2 ng/ml.
For plasma, dilution ratio is 1:1 and concentration is 0.14-1.22 ng/ml..
Some articles we found to cite concentrations of MMP-1 in samples: 25667666 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 215 | 1557 | 4510 | 235 | 1708 | 4065 |
| Standard deviation | 13.97 | 118.33 | 261.58 | 19.5 | 160.55 | 308.94 |
| CV (%) | 6.5% | 7.6% | 5.8% | 8.3% | 9.4% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 215 | 203 | 207 | 218 | 210 | 6.01 | 2.8% |
| Sample 2 | 1557 | 1530 | 1547 | 1360 | 1498 | 80.54 | 5.3% |
| Sample 3 | 4510 | 5168 | 4653 | 4926 | 4814 | 253.07 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For MMP1 (Source: Uniprot.Org, NCBI)
Gene Name
MMP1
Full Name
Interstitial collagenase
Weight
46266 MW
Superfamily
peptidase M10A family
Alternative Names
cDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7) ; MMP1 CLG, CLGN matrix metallopeptidase 1 interstitial collagenase|fibroblast collagenase|matrix metallopeptidase 1 (interstitial collagenase)|matrix metalloprotease 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP1, check out the MMP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MMP-1 ELISA Kit PicoKine® (EK0458)
Hello CJ!
EK0458 has been cited in 36 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts
MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway
Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging
Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia
A Combination of Celecoxib and Glucosamine Sulfate Has Anti-Inflammatory and Chondroprotective Effects: Results from an In Vitro Study on Human Osteoarthritic Chondrocytes
MMP-2, MMP-9, TIMP-1, and TIMP-2 in the Peripheral Blood of Patients with Differentiated Thyroid Carcinoma
Protective Effects of Ginseng Proteins on Photoaging of Mouse Fibroblasts Induced by UVA
Effects of l-Carnitine Supplementation on Serum Inflammatory Factors and Matrix Metalloproteinase Enzymes in Females with Knee Osteoarthritis: A Randomized, Double-Blind, Placebo-Controlled Pilot Study
Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration
Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Human MMP-1 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human MMP-1 Picokine® ELISA Kit (Catalog # EK0458)?
A. Williams
Verified customer
Asked: 2019-11-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of MMP-1 causing degradation of its protein structure. MMP-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the MMP-1 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-15
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring MMP-1 using the EK0458 Human MMP-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-04-05
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Campbell
Verified customer
Asked: 2018-09-04
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-09-04
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-08-28
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-08-28
Question
Q: how to proceed with the analysis of ELISA data? I have performed MMP-1 level in serum.
Verified Customer
Verified customer
Asked: 2018-03-25
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-03-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-02-12
Question
Q: which procedure should I follow in order to thaw whole blood sample for MMP-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-09-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-09-04
Question
Q: Can MMP-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Anand
Verified customer
Asked: 2016-05-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-05-26
Question
Q: What is the optimal O.D. value for MMP-1 ELISA kit? I used your MMP-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MMP-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2014-09-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-1 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of MMP-1 that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2014-09-30
Question
Comparing to your ELISA kits in general, is there any deviation of your standard in this kit? If yes, what is it?
Verified Customer
Verified customer
Asked: 2014-09-11
Answer
Yes. The expression system of the standard in this kit is mouse myeloma cell line, NS0-derived.
Boster Scientific Support
Answered: 2014-09-11
