Human MEP1A ELISA Kit PicoKine®

Product Name

Human MEP1A ELISA Kit PicoKine®

View all Meprin alpha Subunit/MEP1A ELISA kits

SKU/Catalog Number

EK1552

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MEP1A ELISA Kit PicoKine® (96 Tests). Quantitate Human MEP1A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human MEP1A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1552)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: V22-Q601

Sensitivity

<50 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Standard Dilution Instructions

See datasheet of EK1552 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1552 is reactive to MEP1A in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1552 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Meprin alpha Subunit/MEP1A

Meprin A subunit alpha also known as endopeptidase-2 or PABA peptide hydrolase is the alpha subunit of the meprin A enzyme that in humans is encoded by the MEP1A gene. Meprin A subunit alpha, encoded by the **MEP1A** gene, is a component of the meprin A metalloproteinase complex, which is involved in extracellular matrix remodeling and proteolysis. Meprin A is a zinc-dependent endopeptidase found predominantly in the kidneys, intestines, and other tissues. It plays a crucial role in various physiological processes, including inflammation, tissue repair, and the processing of bioactive peptides. The alpha subunit, specifically, is a part of the hetero-oligomeric enzyme complex, which combines with the beta subunit to form an active enzyme. Abnormalities in meprin A function have been associated with several pathological conditions, including kidney diseases and cancer.

Kit Components

Catalog Number	Description	Quantity
EK1552-CAP	Anti-Human MEP1A Pre-coated 96-well strip microplate	1
EK1552-ST	Human MEP1A Standard	2 vials, 100 ng/tube
EK1552-DA	Human MEP1A Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.022	0.137	0.246	0.456	0.747	1.266	1.891	2.028

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MEP1A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Meprin alpha Subunit/MEP1A in ELISA kits from four different production batches/lots.

Gene/Protein Information For MEP1A (Source: Uniprot.Org, NCBI)

Alternative Names

bA268F1.1 (meprin A alpha (PABA peptide hydrolase)); EC 3.4.24; EC 3.4.24.18; endopeptidase-2; MEP1A; meprin A subunit alpha; meprin A, alpha (PABA peptide hydrolase); Meprin alpha Subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA MEP1A PPHA meprin A subunit alpha meprin A subunit alpha|N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha|PABA peptide hydrolase|PPH alpha|bA268F1.1 (meprin A alpha (PABA peptide hydrolase))|endopeptidase-2|meprin A, alpha (PABA peptide hydrolase)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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