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Product Info Summary
| SKU: | EK1837 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human LYPD3 ELISA Kit PicoKine®
View all C4.4A/LYPD3 ELISA kits
SKU/Catalog Number
EK1837
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LYPD3 ELISA Kit PicoKine® (96 Tests). Quantitate Human LYPD3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LYPD3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1837)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L31-C326
Sensitivity
<0.1 ng/ml
Assay Range
0.312 ng/ml - 20 ng/ml
Standard Dilution Instructions
See datasheet of EK1837 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1837 is reactive to LYPD3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1837 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of C4.4A/LYPD3
Ly6/PLAUR domain-containing protein 3 is a protein that in humans is encoded by the LYPD3 gene. This gene is mapped to 19q13.31.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1837-CAP | Anti-Human LYPD3 Pre-coated 96-well strip microplate | 1 |
| EK1837-ST | Human LYPD3 Standard | 2 vials, 20 ng/tube |
| EK1837-DA | Human LYPD3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.3 | 0.63 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.001 | 0.201 | 0.350 | 0.599 | 0.929 | 1.351 | 1.725 | 2.196 |
Data Example Images
Click image to see more details
Human LYPD3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect C4.4A/LYPD3, Dilution ratio of 1:10, concentration in serum and plasma is 5-8 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LYPD3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 590 | 2019 | 10613 | 621 | 2312 | 11100 |
| Standard deviation | 25.96 | 102.97 | 583.72 | 40.99 | 171.09 | 843.60 |
| CV (%) | 4.4% | 5.1% | 5.5% | 6.6% | 7.4% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of C4.4A/LYPD3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 590 | 572 | 604 | 542 | 577 | 23.17 | 4.0% |
| Sample 2 | 2019 | 2207 | 2161 | 2108 | 2123 | 69.89 | 3.2% |
| Sample 3 | 10613 | 9014 | 10567 | 8909 | 9775 | 815.25 | 8.3% |
Protein Target Info & Infographic
Gene/Protein Information For LYPD3 (Source: Uniprot.Org, NCBI)
Gene Name
LYPD3
Full Name
Ly6/PLAUR domain-containing protein 3
Weight
35.971kDa
Alternative Names
Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Matrigel-induced gene C4 protein; MIG-C4; LYPD3; C4.4A LYPD3 C4.4A LY6/PLAUR domain containing 3 ly6/PLAUR domain-containing protein 3|2310061G07Rik|GPI-anchored metastasis-associated protein C4.4A homolog|GPI-anchored metastasis-associated protein homolog|MIG-C4|matrigel-induced gene C4 protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LYPD3, check out the LYPD3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LYPD3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LYPD3 ELISA Kit PicoKine® (EK1837)
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Customer Q&As
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8 Customer Q&As for Human LYPD3 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-09-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-09-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-05-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-05-05
Question
Q: how can I thaw whole blood sample for C4.4A ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test C4.4A for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-02-06
Question
Q: What is the optimal O.D. value for C4.4A ELISA kit? I performed your C4.4A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain C4.4A even though the O.D. values are not very high?
H. Dhawan
Verified customer
Asked: 2018-12-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this C4.4A ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of C4.4A that can be declared/interpreted as positive by the above standard is 0.1ng/ml.
Boster Scientific Support
Answered: 2018-12-24
Question
Q: how to analyze ELISA data? I measured C4.4A level in plasma.
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-30
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-15
Question
Q: can I use heparin plasma as samples in Human C4.4A Picokine® ELISA Kit (Catalog # EK1837)?
Verified Customer
Verified customer
Asked: 2016-08-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of C4.4A causing disruption of its protein structure. C4.4A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the C4.4A ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-08-25
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
B. Hernandez
Verified customer
Asked: 2014-04-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-04-02
