Anti-Nanos homolog 3 Nanos3 Antibody

Nanos3 antibody

Boster Bio Anti-Nanos homolog 3 Nanos3 Antibody (Catalog # A07449). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07449
Size: 0.1 mg
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, WB

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Product Name

Anti-Nanos homolog 3 Nanos3 Antibody

View all Nanos3 Antibodies

SKU/Catalog Number

A07449

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Nanos homolog 3 Nanos3 Antibody (Catalog # A07449). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Nanos3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Nanos homolog 3 Nanos3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07449)

Host

Rabbit

Contents

Nanos3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Nanos3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3. The immunogen is located within the last 50 amino acids of Nanos3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.

Reactive Species

A07449 is reactive to NANOS3 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

18844 MW

Background of Nanos3

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07449 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Nanos3 antibody can be used for detection of Nanos3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For NANOS3 (Source: Uniprot.org, NCBI)

Gene Name

NANOS3

Full Name

Nanos homolog 3

Weight

18844 MW

Superfamily

nanos family

Alternative Names

NOS3, NANOS1L, NOS3, Nanos homolog 3, NOS-3 Nanos3|n, nos3|nanos C2HC-type zinc finger 3|nanos homolog 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NANOS3, check out the NANOS3 Infographic

NANOS3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NANOS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A07449-0.1 mg

0.1 mg

$449

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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