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Product Info Summary
| SKU: | EK2162 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Langerin/CD207 ELISA Kit PicoKine®
View all Langerin/CD207 ELISA kits
SKU/Catalog Number
EK2162
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Langerin/CD207 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD207 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Langerin/CD207 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2162)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: P65-P328
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2162 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2162 is reactive to CD207 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2162 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Langerin/CD207
Langerin (CD207) is a type II transmembrane protein which is encoded by the CD207 gene in humans. The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2162-CAP | Anti-Human CD207 Pre-coated 96-well strip microplate | 1 |
| EK2162-ST | Human CD207 Standard | 2 vials, 10 ng/tube |
| EK2162-DA | Human CD207 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.003 | 0.067 | 0.126 | 0.245 | 0.563 | 0.988 | 1.563 | 2.191 |
Data Example Images
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Human Langerin/CD207 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Langerin/CD207 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 185 | 1366 | 5456 | 183 | 1469 | 5812 |
| Standard deviation | 8.88 | 88.79 | 349.18 | 12.63 | 113.11 | 412.65 |
| CV(%) | 4.8% | 6.5% | 6.4% | 6.9% | 7.7% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Langerin/CD207 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 185 | 195 | 198 | 205 | 193 | 10.96 | 5.6% |
| Sample 2 | 1366 | 1296 | 1142 | 1223 | 1257 | 74.15 | 5.9% |
| Sample 3 | 5456 | 6236 | 5869 | 57415 | 5826 | 262.15 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For CD207 (Source: Uniprot.Org, NCBI)
Gene Name
CD207
Full Name
C-type lectin domain family 4 member K
Weight
Alternative Names
CD207 antigen; CD207 antigen, langerin; CD207 molecule, langerin; CD207; CLEC4KLangerhans cell specific c-type lectin; C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; Langerin CD207 CLEC4K CD207 molecule C-type lectin domain family 4 member K|C-type lectin domain family 4, member K|CD207 antigen, langerin|CD207 molecule, langerin|Langerhans cell specific c-type lectin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD207, check out the CD207 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD207: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Langerin/CD207 ELISA Kit PicoKine® (EK2162)
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