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Product Info Summary
| SKU: | EK0395 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human IL1R1/Il 1 Ri ELISA Kit PicoKine®
SKU/Catalog Number
EK0395
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL1R1/Il 1 Ri ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1R1 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL1R1/Il 1 Ri ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0395)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D21-T332
Sensitivity
<10 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0395 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0395 is reactive to IL1R1 in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0395 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-1 RI
Interleukin 1 receptor, type I (IL1R1), also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0395-CAP | Anti-Human IL1R1 Pre-coated 96-well strip microplate | 1 |
| EK0395-ST | Human IL1R1 Standard | 2 vials, 10 ng/tube |
| EK0395-DA | Human IL1R1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.088 | 0.123 | 0.199 | 0.307 | 0.484 | 0.829 | 1.454 | 2.011 |
Data Example Images
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Human IL1R1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1 RI, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 12.5 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL1R1/Il 1 Ri ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 188 | 973 | 4770 | 205 | 1005 | 4361 |
| Standard deviation | 8.46 | 63.24 | 195.57 | 10.86 | 77.38 | 248.57 |
| CV (%) | 4.5% | 6.5% | 4.1% | 5.3% | 7.7% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1 RI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 188 | 179 | 191 | 173 | 182 | 7.15 | 3.9% |
| Sample 2 | 973 | 977 | 935 | 964 | 962 | 16.42 | 1.7% |
| Sample 3 | 4770 | 4715 | 5460 | 5318 | 5065 | 327.7 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For IL1R1 (Source: Uniprot.Org, NCBI)
Gene Name
IL1R1
Full Name
Interleukin-1 receptor type 1
Weight
65402 MW
Superfamily
interleukin-1 receptor family
Alternative Names
Interleukin-1 receptor type 1;IL-1R-1;IL-1RT-1;IL-1RT1;CD121 antigen-like family member A;Interleukin-1 receptor alpha;IL-1R-alpha;Interleukin-1 receptor type I;p80;CD121a;Interleukin-1 receptor type 1, membrane form;mIL-1R1;mIL-1RI;Interleukin-1 receptor type 1, soluble form;sIL-1R1;sIL-1RI;IL1R1;IL1R, IL1RA, IL1RT1; IL1R1 CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80 interleukin 1 receptor type 1 interleukin-1 receptor type 1|CD121 -like family member A|IL-1R-1|IL-1RT-1|IL-1RT1|interleukin 1 receptor alpha, type I|interleukin-1 receptor alpha|interleukin-1 receptor type I
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL1R1, check out the IL1R1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL1R1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL1R1/Il 1 Ri ELISA Kit PicoKine® (EK0395)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human IL1R1/Il 1 Ri ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-11-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-11-19
Question
Q: how to analyze ELISA data? I have obtained IL-1 RI level in serum.
Verified Customer
Verified customer
Asked: 2020-08-21
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-19
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-09-09
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-1 RI using the EK0395 Human IL-1 RI Picokine® ELISA Kit?
M. Gonzalez
Verified customer
Asked: 2019-07-24
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-07-24
Question
Q: Can IL-1 RI ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-29
Question
Q: if the enzyme conjugated IL-1 RI antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 RI antigen?
H. Scott
Verified customer
Asked: 2017-09-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-09-09
Question
Q: What is the optimal O.D. value for IL-1 RI ELISA kit? I performed your IL-1 RI ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-1 RI even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2015-03-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1 RI ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-1 RI that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2015-03-17
