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Product Info Summary
| SKU: | EK0777 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®
SKU/Catalog Number
EK0777
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine® (96 Tests). Quantitate Human IL6R in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0777)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L20-D358
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0777 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0777 is reactive to IL6R in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
EK0777 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Il6r
IL6R alpha (IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0777-CAP | Anti-Human IL6R Pre-coated 96-well strip microplate | 1 |
| EK0777-ST | Human IL6R Standard | 2 vials, 10 ng/tube |
| EK0777-DA | Human IL6R Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.012 | 0.107 | 0.200 | 0.323 | 0.694 | 1.149 | 1.521 | 2.026 |
Data Example Images
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Human IL-6R alpha PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Il6r, Dilution ratio of 1:100, concentration in serum and plasma is 10837-53930 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 54 | 271 | 821 | 59 | 259 | 773 |
| Standard deviation | 2.86 | 17.07 | 41.87 | 3.77 | 17.35 | 44.06 |
| CV (%) | 5.3% | 6.7% | 5.7% | 6.4% | 6.7% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Il6r in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 54 | 48 | 54 | 48 | 51 | 3 | 5.8% |
| Sample 2 | 271 | 254 | 262 | 234 | 255 | 13.66 | 5.3% |
| Sample 3 | 821 | 928 | 965 | 877 | 897 | 54.21 | 6% |
Protein Target Info & Infographic
Gene/Protein Information For IL6R (Source: Uniprot.Org, NCBI)
Gene Name
IL6R
Full Name
Interleukin-6 receptor subunit alpha
Weight
51548 MW
Superfamily
type I cytokine receptor family
Alternative Names
Interleukin 6 receptor ;Interleukin 6 receptor, isoform CRA_a ;IL6R ;hCG_1810911 ; IL6R CD126, HIES5, IL-1Ra, IL-6R, IL-6R-1, IL-6RA, IL6Q, IL6QTLA, IL6RQ, gp80, IL6R interleukin 6 receptor interleukin-6 receptor subunit alpha|CD126 |IL-6 receptor subunit alpha|membrane glycoprotein 80
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL6R, check out the IL6R Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL6R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine® (EK0777)
Hello CJ!
EK0777 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Beta-Lapachone Attenuates BMSC-Mediated Neuroblastoma Malignant Transformation by Inhibiting Gal-3/Gal-3BP/IL6 Axis
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-12-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-15
Question
Q: which procedure should I follow in order to thaw whole blood sample for Il6r ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-08-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Il6r for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-08-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-03-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-22
Question
Q: Can Il6r ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-16
Question
The protocol said 100µl/sample is needed to run the ELISA kit. However, the volumes of our samples are small and we are just wondering if 50µl/sample instead of 100µl/sample would work with the kit or not?
Verified customer
Asked: 2019-03-11
Answer
There are two solutions for insufficient sample volume for the Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine™ (EK0777), 1.Diluent the samples with the provided sample diluent buffer into 100ul. 2.Add 50ul of standard solution as well, if 50ul of sample will be added into a well.
Boster Scientific Support
Answered: 2019-03-12
Question
Q: can you recommend the dilution ratio of serum samples for detection of Il6r in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Il6r ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Il6r using the EK0777 Human Il6r Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-03-29
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2015-06-13
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-06-13
Question
Q: how do I analyze ELISA data? I measured Il6r level in urine.
Verified Customer
Verified customer
Asked: 2014-05-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-05-20
Question
Youngseok Song 6576 7243 I am a researcher from Singapore Eye Research Institute. I have a query regarding Human IL-6R Alpha / IL 6 Receptor PicoKine
Verified Customer
Verified customer
Asked: 2014-01-07
Answer
There are two solutions for insufficient sample volume. 1.Diluent the samples with the provided sample diluent buffer into 100ul. 2.Add 50ul of standard solution as well if 50ul of sample will be added into a well.
Boster Scientific Support
Answered: 2014-01-07
