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Product Info Summary
| SKU: | EK0410 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
Product info
Product Name
Human IL-6/Interleukin-6 ELISA Kit PicoKine®
SKU/Catalog Number
EK0410
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-6/Interleukin-6 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-6/Interleukin-6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0410)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: P29-M212
Sensitivity
<0.3 pg/ml
Assay Range
4.69 pg/ml - 300 pg/ml
Standard Dilution Instructions
See datasheet of EK0410 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0410 is reactive to IL6 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Application Guarantee
EK0410 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-6
The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0410-CAP | Anti-Human IL6 Pre-coated 96-well strip microplate | 1 |
| EK0410-ST | Human IL6 Standard | 2 vials, 10 ng/tube |
| EK0410-DA | Human IL6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 4.69 | 9.38 | 18.75 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.031 | 0.091 | 0.127 | 0.222 | 0.513 | 0.749 | 1.186 | 1.997 |
Data Example Images
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Human IL-6 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-6, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 4.69 pg/ml..
Some articles we found to cite concentrations of IL-6 in samples: 24401617 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-6/Interleukin-6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 10 | 38 | 145 | 9 | 37 | 130 |
| Standard deviation | 0.62 | 1.67 | 10.73 | 0.64 | 1.66 | 11.18 |
| CV (%) | 6.2% | 4.4% | 7.4% | 7.2% | 4.5% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 10 | 12 | 11 | 10 | 10 | 0.82 | 8.2% |
| Sample 2 | 38 | 43 | 40 | 42 | 40 | 1.92 | 4.8% |
| Sample 3 | 145 | 149 | 145 | 150 | 147 | 2.27 | 1.5% |
Protein Target Info & Infographic
Gene/Protein Information For IL6 (Source: Uniprot.Org, NCBI)
Gene Name
IL6
Full Name
Interleukin-6
Weight
23,718 MW
Superfamily
IL-6 superfamily
Alternative Names
Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2 IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL6, check out the IL6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-6/Interleukin-6 ELISA Kit PicoKine® (EK0410)
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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20 Customer Q&As for Human IL-6/Interleukin-6 ELISA Kit PicoKine®
Question
Is EK0410 valid for human milk samples?
Verified customer
Asked: 2022-03-30
Answer
Human IL-6/Interleukin-6 ELISA Kit PicoKine® (EK0410) has not been tested on human milk explicitly. In our experience customers have used it successfully in human milk. Thus we are willing to extend our quality guarantee to cover human milk as a viable sample type.
Boster Scientific Support
Answered: 2022-03-30
Question
Is EK0410 still usable if left at room temperature without cooling for 2 days?
Verified customer
Asked: 2020-06-18
Answer
The Human IL-6 ELISA Kit PicoKine™ (EK0410) should be stable but it is not covered by warranty if not stored according to instructions.
Boster Scientific Support
Answered: 2020-06-18
Question
Hello Support: I purchased Human IL-6 ELISA Kit but I have some questions, could you please give advice on staining technique? Is it possible to use only few wells for analysis of several samples and save other plates for further studies? Lastly, do I need to buy any software for data analysis, or Excel will be enough?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The Human IL-6 ELISA Kit comes with 96 well with removable strips, so you can remove as many strips as you need before using it, then store the remaining strips in the pouch with desiccant, sealed it tightly and put in storage at 4C. For your data analysis excel will do. All you need to do is to calculate the average absorbance values and create a standard curve, then interpolate the sample concentrations from the standard curve.
Boster Scientific Support
Answered: 2020-04-30
Question
Q: can I use heparin plasma as samples in Human IL-6 Picokine® ELISA Kit (Catalog # EK0410)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-6 causing disruption of its protein structure. IL-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-6 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-20
Question
What is the expiration date of EK0525 and EK0410 from the time of manufacturing, 6 months or 1 year?
Verified customer
Asked: 2019-12-17
Answer
Expiration is based on storage temperature. Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Boster Scientific Support
Answered: 2019-12-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-22
Question
I purchased Human IL-6 ELISA Kit and I reconstitute with acetic acid and the coating buffer is alkaline solution of pH 9.6. I am concern with the pH change and possibly affects my results? Please let me know
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We recommend to use for coating buffers, the standard PBS rather than alkaline carbonate buffers for this antibody Human IL-6 ELISA Kit
Boster Scientific Support
Answered: 2019-08-30
Question
Is the Human IL-6 ELISA Kit have been tested with saliva samples? Do you have any suggestions?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The Human IL-6 ELISA Kit is suitable to use in saliva samples and the usual protocol would apply. No need for pre-treatment of sample before assay.
Boster Scientific Support
Answered: 2019-08-15
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-04
Question
What is the normal serum range of EK0410?
Verified customer
Asked: 2019-04-08
Answer
Please see the normal serum range for the Human IL-6 ELISA Kit PicoKine™ (EK0410) below; IL-6 (Catlog no. EK0410), 0-15pg/mL
Boster Scientific Support
Answered: 2019-04-09
Question
Is it normal to have fluid in the well of the EK0410 plate and a solid standard that is very hard to dissolve.
Verified customer
Asked: 2019-04-01
Answer
For the Human IL-6 ELISA Kit PicoKine™ (EK0410), it is normal that there is fluid in the wells of the plate, and the drops could be removed by patting the plate on a paper towel or by aspiration. For the standard, we never encountered this problem before, and our technicians suggests to centrifuge at 2000-4000×g for 5 minutes after dissolving the standard.
Boster Scientific Support
Answered: 2019-04-02
Question
Have you tested EK0410 and EK0416 in tissue homogenates and Cell culture supernates?
Verified customer
Asked: 2019-03-15
Answer
The Human IL-6 EK0410 Elisa Kit and Human IL-10 EK0416 Elisa Kit have not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-03-18
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-6 using the EK0410 Human IL-6 Picokine® ELISA Kit?
E. Puri
Verified customer
Asked: 2019-03-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-03-11
Question
Please let me know if you CAN supply QC materials for EACH of these kits?. If you could introduce in EACH kit at least 2 or 3 vials with QC material (having 2 or 3 different concentrations of the analyte for which the kits are used) it would be PERFECT! (even if the raw materials of these QC vials will be the same as the standards
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
We can not provide 2 or 3 different concentrations of the analyte(e.g. serum) for those kits. The QC vials we can provide are recombinant proteins of different concentrtaions.
Boster Scientific Support
Answered: 2019-02-12
Question
Does EK0410 work with whole cell lysates?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
EK0410 is not suitable for working with whole cell lysates.
Boster Scientific Support
Answered: 2018-06-29
Question
Have you tested this kit with pig samples? Keyword: applications, porcine, detection
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
No, we have not tested this with pig samples. A pilot experiment is recommended first if you plan to detect pig samples.
Boster Scientific Support
Answered: 2018-02-12
Question
What are the minimum amounts of blood that must be collected to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF? I'd like to know the necessary amounts for each analysis done singly. Keyword: protocol, applications
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
0.1ml sample will be added into each well, so you need to collect 0.6ml of sample at most if you plan to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF. And the sample needs to be diluted for the analysis of ICAM, VCAM.
Boster Scientific Support
Answered: 2017-09-13
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Harris
Verified customer
Asked: 2017-07-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-07-23
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IL-6 level in plasma.
Verified Customer
Verified customer
Asked: 2016-07-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-07-29
Question
We received an inquiry regarding the EK0410 IL-6 ELISA Kit (Human) from a customer:
Verified Customer
Verified customer
Asked: 2016-01-06
Answer
<0.3pg/ml *The sensitivity or the minimum detectable dose (MDD) is the lower limit of target protein that can be detected by the kit. It is determined by adding two standard deviations to the mean O.D. value of twenty (20) blank wells and calculating the corresponding concentration.
Boster Scientific Support
Answered: 2016-01-06
