Click image to see more details
Product Info Summary
| SKU: | EK0785 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IL-17RB ELISA Kit PicoKine®
SKU/Catalog Number
EK0785
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-17RB ELISA Kit PicoKine® (96 Tests). Quantitate Human IL17RB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-17RB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0785)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: R18-K286
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0785 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0785 is reactive to IL17RB in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0785 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-17RB
IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0785-CAP | Anti-Human IL17RB Pre-coated 96-well strip microplate | 1 |
| EK0785-ST | Human IL17RB Standard | 2 vials, 10 ng/tube |
| EK0785-DA | Human IL17RB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.038 | 0.134 | 0.203 | 0.394 | 0.664 | 1.146 | 1.952 | 2.669 |
Data Example Images
Click image to see more details
Human IL-17RB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-17RB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 303 | 1191 | 57321 | 321 | 1173 | 5862 |
| Standard deviation | 14.54 | 47.64 | 411.44 | 17.01 | 58.65 | 527.58 |
| CV (%) | 4.8% | 4% | 7.1% | 5.3% | 5% | 9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-17RB in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 303 | 331 | 321 | 324 | 319 | 10.32 | 3.2% |
| Sample 2 | 1191 | 1271 | 1261 | 1276 | 1249 | 34.34 | 2.7% |
| Sample 3 | 5795 | 5828 | 6134 | 6129 | 5971 | 160.43 | 2.6% |
Protein Target Info & Infographic
Gene/Protein Information For IL17RB (Source: Uniprot.Org, NCBI)
Gene Name
IL17RB
Full Name
Interleukin-17 receptor B
Weight
55885 MW
Alternative Names
Interleukin-17 receptor B;IL-17 receptor B;IL-17RB;Cytokine receptor-like 4;IL-17 receptor homolog 1;IL-17Rh1;IL17Rh1;Interleukin-17B receptor;IL-17B receptor;IL17RB;CRL4, EVI27, IL17BR;UNQ2501/PRO19612; Il17rb|Evi, Evi27, IL-1, IL-17, IL-17ER, IL-17Rh1, IL17, IL17RH1, Il1, Il17br|interleukin 17 receptor B|interleukin-17 receptor B|IL-17 receptor B|IL-17 receptor homolog 1|IL-17B receptor|IL-17RB|ecotropic viral integration site 27|interleukin 17B receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL17RB, check out the IL17RB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL17RB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-17RB ELISA Kit PicoKine® (EK0785)
Hello CJ!
No publications found for EK0785
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human IL-17RB ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human IL-17RB ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Human IL-17RB ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-10-23
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-17RB in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
P. Patel
Verified customer
Asked: 2019-10-20
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-17RB ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-10-20
Question
Q: is it okay to use citrate plasma as samples in Human IL-17RB Picokine® ELISA Kit (Catalog # EK0785)?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-17RB causing degradation of its protein structure. IL-17RB may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-17RB ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-09-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Evans
Verified customer
Asked: 2018-11-11
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-11
Question
Q: how to proceed with the analysis of ELISA data? I measured IL-17RB level in cell lysates.
M. Davis
Verified customer
Asked: 2018-09-23
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-09-23
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-17RB using the EK0785 Human IL-17RB Picokine® ELISA Kit?
H. Parker
Verified customer
Asked: 2018-01-15
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-01-15
