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Product Info Summary
| SKU: | EK2216 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human HNMT ELISA Kit PicoKine®
View all Histamine N-Methyltransferase/HNMT ELISA kits
SKU/Catalog Number
EK2216
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human HNMT Kit PicoKine™ (96 Tests). Quantitate Human HNMT in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human HNMT ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2216)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A2-A292
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2216 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2216 is reactive to HNMT in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2216 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Histamine N-Methyltransferase/HNMT
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2216-CAP | Anti-Human HNMT Pre-coated 96-well strip microplate | 1 |
| EK2216-ST | Human HNMT Standard | 2 vials, 10 ng/tube |
| EK2216-DA | Human HNMT Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.049 | 0.072 | 0.119 | 0.198 | 0.421 | 0.665 | 1.278 | 2.012 |
Data Example Images
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Human HNMT PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Histamine N-Methyltransferase/HNMT, Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human HNMT ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 113 | 684 | 1463 | 110 | 702 | 1532 |
| Standard deviation | 4.41 | 34.88 | 70.22 | 5.72 | 31.59 | 78.13 |
| CV (%) | 3.9% | 5.1% | 4.8% | 5.2% | 4.5% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Histamine N-Methyltransferase/HNMT in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 113 | 121 | 118 | 109 | 115 | 4.15 | 6.1% |
| Sample 2 | 684 | 721 | 706 | 698 | 702 | 42.84 | 6.1% |
| Sample 3 | 1463 | 1522 | 1469 | 1463 | 1479 | 72.48 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For HNMT (Source: Uniprot.Org, NCBI)
Gene Name
HNMT
Full Name
Histamine N-methyltransferase
Weight
33.295kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
EC 2.1.1.8; Histamine NMethyltransferase; Histamine N-Methyltransferase; HMT; HNMT; HNMT-S1; HNMT-S2 HNMT HMT-S1, HNMT-S2, MRT51, HNMT histamine N-methyltransferase histamine N-methyltransferase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on HNMT, check out the HNMT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HNMT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human HNMT ELISA Kit PicoKine® (EK2216)
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