Human GSTM1 ELISA Kit PicoKine®

Product Name

Human GSTM1 ELISA Kit PicoKine®

View all GSTM1 ELISA kits

SKU/Catalog Number

EK2213

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GSTM1 Kit PicoKine® (96 Tests). Quantitate Human GSTM1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GSTM1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2213)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: M1-K218

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

See datasheet of EK2213 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2213 is reactive to GSTM1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2213 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GSTM1

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.

Kit Components

Catalog Number	Description	Quantity
EK2213-CAP	Anti-Human GSTM1 Pre-coated 96-well strip microplate	1
EK2213-ST	Human GSTM1 Standard	2 vials, 10 ng/tube
EK2213-DA	Human GSTM1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.024	0.066	0.128	0.247	0.491	0.860	1.452	2.019

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GSTM1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GSTM1 in ELISA kits from four different production batches/lots.

Gene/Protein Information For GSTM1 (Source: Uniprot.Org, NCBI)

Alternative Names

EC 2.5.1.18; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; HB subunit 4; H-B; MGC26563; MU; MU-1; S-(hydroxyalkyl)glutathione lyase GSTM1 GST1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU, MU-1, GSTM1 glutathione S-transferase mu 1 glutathione S-transferase Mu 1|GST HB subunit 4|GST class-mu 1|HB subunit 4|S-(hydroxyalkyl)glutathione lyase|glutathione S-alkyltransferase|glutathione S-aralkyltransferase|glutathione S-aryltransferase|glutathione S-transferase M1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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