GSTM1 Antibodies

Glutathione S-transferase Mu 1 (GSTM1)

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. .

Gene Information

Human
Gene Name:	GSTM1
Uniprot:	P09488
Entrez:	2944

Family

Belongs to:
GST superfamily

Alternative Names

EC 2.5.1.18; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; HB subunit 4; H-B; MGC26563; MU; MU-1; S-(hydroxyalkyl)glutathione lyase

Molecular Weight

Mass (kDA):
25.712 kDA

Genomic Context

Human
Location:	1p13.3
Sequence:	1; NC_000001.11 (109687817..109693745)

Tissue Specificity

Liver (at protein level).

Subcellular Localizations

Cytoplasm.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Malignant Neoplasm Of Lung
• Lung Neoplasms
• Malignant Squamous Cell Neoplasm
• Carcinogenesis
• Malignant Neoplasm Of Breast
• Malignant Neoplasm Of Urinary Bladder
• Mammary Neoplasms
• Leukemia
• Bladder Neoplasm
• Adenocarcinoma

• Colorectal Cancer
• Colorectal Neoplasms
• Asthma
• 5,10-methylenetetrahydrofolate Reductase Deficiency
• Cytogenetic Abnormality
• Malignant Paraganglionic Neoplasm
• Stomach Neoplasms

Pathways

• Dna Repair
• Pathogenesis
• Conjugation
• Excretion
• Methylation
• Transport
• Inflammatory Response
• Aging
• Cell Proliferation
• Dna Methylation
• Cell Cycle
• Response To Oxidative Stress
• Mismatch Repair

• Cell Death
• Hypersensitivity
• Localization
• Drug Resistance
• Reverse Transcription
• Spermatogenesis
• Menarche

PTMs

• Acetylation
• Methylation
• Oxidation
• Reduction

• Phosphorylation
• Hydroxylation
• Phosphorothioation
• Glutathionylation

• Glycosylation

Research Areas

• Epitope Tags

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GSTM1

Boster Bio - Best Uses Of The GSTM1 Marker

A lot of scientists have been wondering how to maximize the value of the GSTM1 marker. Boster offers a variety of choices for this particular protein. There are ELISA kits and monoclonal anti-GST3/GSTp1 antibody and PCR-RFLP methods to determine GSTP1 polymorphism. This article will cover the various options available and how to best keep this marker.

Monoclonal Anti-GST3/GSTp1 antibody

The Boster Bio Anti GST3/GSTRp1 Monoclonal Antibody recognizes mouse, human, and the rat GST3 protein sequences. It also detects cleaved caspase-9. The product is designed to be highly specific, allowing you to perform research of high-quality and faster. It is suitable for a wide range of applications, including immunohistochemistry, western blot, and ICC.

The company's GSTP1 monoclonal antibodies are created through a proprietary process. A QIAamp Blood Kit was used to extract DNA from blood samples. The DNA was used as a template for an 25 ul solution, which contained Tag(r),Green Master Mix (2x).

ELISA kits

ELISA kits for the GSTM1 protein marker are suitable for quantitative measurement of the GSTM1 protein in serum, plasma or homogenates of tissue. The GSTM1 test is extremely sensitive and specific. The GSTM1 ELISA kit is highly specific, and there is no significant cross-reactivity to analogues. The GSTM1 ELISA Kit needs very low operating conditions such as room temperature and incubator temperature. The entire assay must be conducted by the same person or in the laboratory.

The GSTM1 gene has been linked to various cancer kinds. It has also been linked to poor prognosis, lower survival and lower rates of survival. GSTM1 knockdown significantly decreased the viability of tumor cells and caused the cell cycle to arrest in the G0/G1 cycle. GSTM1 is believed to regulate NF-kB-mediated signaling and STAT3 signaling. Further research will help reveal the molecular mechanism behind the prognostic value of the GSTM1 protein.

An ELISA kit to measure GSTM1 expression is a cost-effective and efficient method of determining its level. Transfected cells will express GSTM1. GSTM1 gene. To conduct an ELISA for GSTM1 in U87 cells, cDNA was seeded in a six-well plate or 12-well. Transfection was done using DMEM that contained 10% FBS.

Various retail labs can sell ELISA kits for GSTM1 protein. Creative Diagnostics offers complete kits with reagents, antibodies and antibody pairs. They are extremely precise and extremely convenient. They can also detect targets in diverse samples. They also detect targets in a variety of samples. ELISA kits include pre-coated plates, standards for capturing and detection of antibodies buffers, and other substances.

ELISA kits for the GSTM1 gene can be used to identify an chromatin gene that has higher accessibility, which has predictive value in GBM tumors. In addition this marker has been confirmed to be a prognostic indicator for patients. Additionally, knockdowns of the GSTM1 gene reduced the viability and cell cycle of cells. It also decreased the phosphorylation levels of STAT3 and NF-kB subunits.

The ELISA kit that measures the GSTM1 marker's level of protein in the sample is an efficient economical, simple and convenient method. The test kit contains antibodies that are specific to the GSTM1 marker. It is highly sensitive and precise. Multiple samples can be analysed simultaneously. The GSTM1 ELISA Kit offers affordable results as well as high-quality. The GSTM1 ELISA Kit is a fantastic option for qualitative analysis.

A PCR-RFLP method to detect GSTP1 polymorphism

The PCR-RFLP approach to detect GSTR1 polymorphisms was originally designed to examine the GSTP1 gene in Iraqi populations. The study employed a combination of both RFLP and PCR to find the polymorphism in a tiny number of Iraqi citizens. The PCR-RFLP technique employs the 176-bp fragment and a restriction fragment length polymorphism (RFLP) method to identify the genotype. The RFLP method detects a G–A shift in the GSTR1 genome.

The PCR-RFLP method was successful in detecting SNPs within the GSTP1 gene. To determine the genotype of the GSTP1 gene and its allele status, we used the Alw261 Primer. For the PCR-RFLP reaction, we used 30 ng genomic DNA for each sample, and 1xPCR buffer as well as 2 mM MgCl2. This method gave three genotypes.

The study revealed that those who had the CYP1A1 "TC" allele were at risk for bladder cancer. These results suggest that the GSTP1c.313A>G polymorphic locus was not a significant risk factor for bladder cancer in Turkish people. These findings should be confirmed by future studies of case series. All participants signed an informed consent, and the study was approved by the Harran University School of Medicine's Ethics Committee.

The PCR-RFLP method to detect GSTM1 polymorphism has numerous advantages over the traditional RFLP method. In addition to detecting GSTM1 polymorphism, PCR-RFLP is highly sensitive and reliable in determining status of methylation in the GSTP1 gene. However, it takes an extensive amount of time and is therefore not recommended for regular use.

The results of the current study are encouraging. The polymorphic variant in GSTP1 has been associated with increased survival rates among patients suffering from breast and colon cancer. These findings could lead to more personalized treatment plans for patients with myeloma depending on their host genetic factors. Further research is required in order to determine the precise effect of this genetic polymorphism.

Storing the GSTM1 Marker

The storage of GSTM1 markers is important due to their link with the risk of developing cancer. These genes are susceptible to an oxidative stress. Oxidative stress can also affect other areas of the blood. This study investigated the possibility that polymorphisms in these enzymes are associated with an increased risk of lung carcinoma. The study involved lung cancer patients and controls from Iran's two referral hospitals. Genomic DNA was extracted from the cancerous tissues of patients and from the blood buffy coats of healthy controls. The multiplex sequencing of these samples was carried out to determine whether the patients were GSTM1 homozygous or null.

The location of the primary tumor as well as the TNM classification of the cancer are two variables that affect the GST genotype. Patients with early-stage cancers are more likely to have a GSTT1*0 genetic profile than those who have the GSTT1*1 genotype. However, patients who are in the later stages of the cancer are more likely to have a GSTT1*1 genotype. These findings underscore the importance of understanding the genetic causes for storage lesions as well as how to improve the care provided to patients through blood transfusion.

The antibody for GSTM1 recognizes both human and non-human GSTM1 proteins. It is produced from goat serum by antigen affinity chromatography. It is recommended to store it at 20°C. Mix the antibody in a gentle manner prior to using. The antibody is not intended to be used as a medication. If you experience adverse reactions you should consult your physician. It is essential to dilute the GSTM1 antibody correctly before using it.

The storage of GSTM1 markers is essential for determining the presence of mutations in the human genome. This is because the deletion of GSTM1 results in an allele that is null. The null allele is associated with unbalanced crossing-over, and could be the result of unequal crossing-over. LC480 is cost-effective and reduces costs. It only requires SYBR Green I. It also removes the need for downstream relative quantification.