Human GPVI ELISA Kit PicoKine®

GPVI ELISA kit for Human

Human GPVI Kit PicoKine® (96 Tests). Quantitate Human GP6 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2212
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human GPVI ELISA Kit PicoKine®

View all GPVI ELISA kits

SKU/Catalog Number

EK2212

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GPVI Kit PicoKine® (96 Tests). Quantitate Human GP6 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GPVI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2212)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q21-K267

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2212 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2212 is reactive to GP6 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2212 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GPVI

Glycoprotein VI (platelet), also known as GPVI, is a glycoprotein receptor for collagen which is expressed in platelets. In humans, glycoprotein VI is encoded by the GPVI gene. This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Kit Components

Catalog Number Description Quantity
EK2212-CAP Anti-Human GP6 Pre-coated 96-well strip microplate 1
EK2212-ST Human GP6 Standard 2 vials, 10 ng/tube
EK2212-DA Human GP6 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0270.0780.1410.2670.4230.9681.3752.062

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GPVI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3891264412333513964226
Standard deviation17.5170.78131.9418.4380.97207.07
CV(%)4.5%5.6%3.2%5.5%5.8%4.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GPVI in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 138937736535837221.965.9%
Sample 21264127512891296128165.335.1%
Sample 341234566469347564535276.66.1%
*number of samples for each test n=16.

Gene/Protein Information For GP6 (Source: Uniprot.Org, NCBI)

Gene Name

GP6

Full Name

Platelet glycoprotein VI

Weight

36.866kDa

Alternative Names

Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI GP6 BDPLT11, GPIV, GPVI glycoprotein VI platelet platelet glycoprotein VI|glycoprotein 6|platelet collagen receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GP6, check out the GP6 Infographic

GP6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GP6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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