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Product Info Summary
| SKU: | EK2209 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human GLO1 ELISA Kit PicoKine®
View all Glyoxalase I ELISA kits
SKU/Catalog Number
EK2209
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GLO1 Kit PicoKine® (96 Tests). Quantitate Human GLO1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GLO1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2209)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A2-M184
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2209 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2209 is reactive to GLO1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2209 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Glyoxalase I
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2209-CAP | Anti-Human GLO1 Pre-coated 96-well strip microplate | 1 |
| EK2209-ST | Human GLO1 Standard | 2 vials, 10 ng/tube |
| EK2209-DA | Human GLO1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.027 | 0.042 | 0.086 | 0.256 | 0.523 | 1.068 | 1.375 | 1.962 |
Data Example Images
Click image to see more details
Human GLO1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Glyoxalase I, Dilution ratio of 1:1, concentration in serum and plasma is around 4 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GLO1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 350 | 1269 | 4565 | 378 | 1366 | 4475 |
| Standard deviation | 19.25 | 65.99 | 164.34 | 19.28 | 61.47 | 214.8 |
| CV(%) | 5.5% | 5.2% | 3.6% | 5.1% | 4.5% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Glyoxalase I in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 350 | 365 | 370 | 368 | 363 | 18.53 | 5.1% |
| Sample 2 | 1269 | 1305 | 1288 | 1283 | 1286 | 46.31 | 3.6% |
| Sample 3 | 4565 | 4541 | 4720 | 4690 | 4629 | 282.37 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For GLO1 (Source: Uniprot.Org, NCBI)
Gene Name
GLO1
Full Name
Lactoylglutathione lyase
Weight
20.778kDa
Superfamily
glyoxalase I family
Alternative Names
Aldoketomutase; EC 4.4.1.5; GLO1; GLOD1; Glx I; GLYI; glyoxalase domain containing 1; Glyoxalase I; glyoxalase Ialdoketomutase; Ketone-aldehyde mutase; lactoyl glutathione lyase; lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase GLO1 GLOD1, GLYI, HEL-S-74 glyoxalase I lactoylglutathione lyase|S-D-lactoylglutathione methylglyoxal lyase|aldoketomutase|epididymis secretory protein Li 74|glx I|glyoxalase domain containing 1|ketone-aldehyde mutase|lactoyl glutathione lyase|methylglyoxalase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GLO1, check out the GLO1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GLO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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