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Product Info Summary
| SKU: | EK1240 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human Galectin-3BP ELISA Kit PicoKine®
View all Galectin-3BP/MAC-2BP/LGALS3BP ELISA kits
SKU/Catalog Number
EK1240
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Galectin-3BP ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS3BP in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Galectin-3BP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1240)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V19-D585
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1240 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1240 is reactive to LGALS3BP in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1240 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Galectin-3BP/MAC-2BP/LGALS3BP
Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Using fluorescence in–situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1240-CAP | Anti-Human LGALS3BP Pre-coated 96-well strip microplate | 1 |
| EK1240-ST | Human LGALS3BP Standard | 2 vials, 10 ng/tube |
| EK1240-DA | Human LGALS3BP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.022 | 0.094 | 0.144 | 0.278 | 0.520 | 0.902 | 1.468 | 2.070 |
Data Example Images
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Human Galectin-3BP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Galectin-3BP/MAC-2BP/LGALS3BP, Dilution ratio of 1:5000, concentration in serum and plasma is 1.7-8.5 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Galectin-3BP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 312 | 1090 | 4158 | 326 | 1154 | 3768 |
| Standard deviation | 18.72 | 63.22 | 282.74 | 20.86 | 84.24 | 271.29 |
| CV (%) | 6% | 5.8% | 6.8% | 6.4% | 7.3% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Galectin-3BP/MAC-2BP/LGALS3BP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 312 | 367 | 361 | 366 | 351 | 22.91 | 6.5% |
| Sample 2 | 1090 | 1067 | 1143 | 1041 | 1085 | 37.57 | 3.4% |
| Sample 3 | 4158 | 3836 | 4350 | 4159 | 4125 | 184.65 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For LGALS3BP (Source: Uniprot.Org, NCBI)
Gene Name
LGALS3BP
Full Name
Galectin-3-binding protein
Weight
65331 MW
Alternative Names
Galectin-3-binding protein;Basement membrane autoantigen p105;Lectin galactoside-binding soluble 3-binding protein;Mac-2-binding protein;MAC2BP;Mac-2 BP;Tumor-associated antigen 90K;LGALS3BP;M2BP; LGALS3BP 90K, BTBD17B, CyCAP, M2BP, MAC-2-BP, TANGO10B, gp90 galectin 3 binding protein galectin-3-binding protein|L3 |MAC2BP|Mac-2-binding protein|basement membrane auto p105|lectin galactoside-binding soluble 3-binding protein|mac-2 BP|serum protein 90K|transport and golgi organization 10 homolog B|tumor-associated 90K
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LGALS3BP, check out the LGALS3BP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS3BP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Galectin-3BP ELISA Kit PicoKine® (EK1240)
Hello CJ!
EK1240 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Multiple coagulation factor deficiency protein 2 as a crucial component in metastasis of human oral cancer
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Human Galectin-3BP ELISA Kit PicoKine®
Question
What are the host, purification and immunogen for Capture Antibody and Detection Antibody ?
Verified customer
Asked: 2022-08-29
Answer
For Capture Ab, 1. Host/Clonality: monoclonal antibody from mouse 2. Purification: Protein A or G Purified 3. Immunogen: aa19-aa585 For Detection Ab, 1. Host/Clonality: monoclonal antibody from mouse 2.Purification: Protein A or G Purified 3. Immunogen: aa19-aa585
Boster Scientific Support
Answered: 2022-08-29
Question
What are the Standard Source Standard Sequence Standard Expression Transfection Method (if applicable) Standard Accession Number?
Verified customer
Asked: 2022-08-29
Answer
Standard Source: Recombinant Protein Standard Sequence: aa19-aa585 Standard Expression: NS0 Expression System Transfection Method (if applicable): N/A Standard Accession Number: Q08380
Boster Scientific Support
Answered: 2022-08-29
Question
1. Are the Streptavidin and HRP recombinant proteins? 2. What is the Expression System?
Verified customer
Asked: 2022-08-29
Answer
1. Streptavidin yes HRP no E. coli Expression System 2. E. coli Expression System
Boster Scientific Support
Answered: 2022-08-29
Question
Sample Types Tested?
Verified customer
Asked: 2022-08-28
Answer
Human cell culture supernatants, serum and plasma (heparin).
Boster Scientific Support
Answered: 2022-08-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
C. Bhasin
Verified customer
Asked: 2020-11-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-11-20
Question
Q: can you recommend the dilution ratio of serum samples for detection of Galectin-3BP in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-3BP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-12-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. Brown
Verified customer
Asked: 2019-12-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-12-24
Question
Is the product Wisteria Floribunda agglutinin positive Mac-2 binding protein Human ELISA Kit the same as Human Galectin-3BP ELISA Kit PicoKine™ (EK1240)?
Verified customer
Asked: 2019-09-04
Answer
For the Human Galectin-3BP ELISA Kit PicoKine™ (EK1240), it is not the same as Wisteria Floribunda agglutinin positive Mac-2 binding protein Human ELISA Kit. The target species are different and will unlikely cross-react.
Boster Scientific Support
Answered: 2019-09-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Galectin-3BP using the EK1240 Human Galectin-3BP Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-08-24
Question
Q: What is the optimal O.D. value for Galectin-3BP ELISA kit? I used your Galectin-3BP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Galectin-3BP even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-3BP ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Galectin-3BP that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-03-26
Question
Q: can I use citrate plasma as samples in Human Galectin-3BP Picokine® ELISA Kit (Catalog # EK1240)?
Verified Customer
Verified customer
Asked: 2017-03-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Galectin-3BP causing disruption of its protein structure. Galectin-3BP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Galectin-3BP ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-03-01
Question
Q: Can Galectin-3BP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-05-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-05-15
