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Product Info Summary
| SKU: | EK2283 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human ECM1 ELISA Kit PicoKine®
View all ECM-1/Secretory Component P85 ELISA kits
SKU/Catalog Number
EK2283
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ECM1 ELISA Kit PicoKine®(96 Tests). Quantitate Human ECM1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ECM1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2283)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A20-E540
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2283 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2283 is reactive to ECM1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2283 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ECM-1/Secretory Component P85
Extracellular matrix protein 1 is a protein that in humans is encoded by the ECM1 gene. This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2283-CAP | Anti-Human ECM1 Pre-coated 96-well strip microplate | 1 |
| EK2283-ST | Human ECM1 Standard | 2 vials, 10 ng/tube |
| EK2283-DA | Human ECM1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.036 | 0.095 | 0.244 | 0.487 | 0.652 | 0.943 | 1.365 | 2.058 |
Data Example Images
Click image to see more details
Human ECM1 ELISA Kit PicoKine standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ECM1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 251 | 1041 | 3816 | 180 | 1013 | 4617 |
| Standard deviation | 18.9 | 32.79 | 140.81 | 13.75 | 27.76 | 211.92 |
| CV(%) | 7.5% | 3.2% | 3.7% | 7.6% | 2.7% | 4.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ECM-1/Secretory Component P85 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 251 | 301 | 306 | 301 | 290 | 17.65 | 6.1% |
| Sample 2 | 1041 | 946 | 960 | 1121 | 1017 | 40.99 | 4% |
| Sample 3 | 3816 | 2746 | 4780 | 2745 | 3522 | 215.88 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For ECM1 (Source: Uniprot.Org, NCBI)
Gene Name
ECM1
Full Name
Extracellular matrix protein 1
Weight
61 kDa
Alternative Names
ECM1; extracellular matrix protein 1; p85; Secretory Component Glycoprotein; Secretory Component P85 ECM1 URBWD extracellular matrix protein 1 extracellular matrix protein 1|secretory component p85|testicular tissue protein Li 61
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ECM1, check out the ECM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ECM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ECM1 ELISA Kit PicoKine® (EK2283)
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