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Product Info Summary
| SKU: | EK2161 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human DNAM-1/CD226 ELISA Kit PicoKine®
View all DNAM-1/CD226 ELISA kits
SKU/Catalog Number
EK2161
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DNAM-1/CD226 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD226 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human DNAM-1/CD226 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2161)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E19-A254
Sensitivity
<5 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2161 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2161 is reactive to CD226 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2161 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DNAM-1/CD226
CD226 (Cluster of Differentiation 226), PTA1 (outdated term, 'platelet and T cell activation antigen 1') or DNAM-1 (DNAX Accessory Molecule-1) is a protein that in humans is encoded by the CD226 gene which is located on chromosome 18q22.3. This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2161-CAP | Anti-Human CD226 Pre-coated 96-well strip microplate | 1 |
| EK2161-ST | Human CD226 Standard | 2 vials, 10 ng/tube |
| EK2161-DA | Human CD226 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.041 | 0.074 | 0.183 | 0.365 | 0.622 | 0.946 | 1.525 | 2.154 |
Data Example Images
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Human DNAM-1/CD226 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DNAM-1/CD226 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 132 | 801 | 3566 | 139 | 741 | 3657 |
| Standard deviation | 5.81 | 48.06 | 206.83 | 3.59 | 52.61 | 241.36 |
| CV(%) | 4.4% | 6.0% | 5.8% | 6.9% | 7.1% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DNAM-1/CD226 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 132 | 155 | 157 | 148 | 148 | 8.29 | 5.6% |
| Sample 2 | 801 | 766 | 780 | 722 | 767 | 36.06 | 4.7% |
| Sample 3 | 3566 | 3941 | 3852 | 3629 | 3747 | 172.36 | 4.65% |
Protein Target Info & Infographic
Gene/Protein Information For CD226 (Source: Uniprot.Org, NCBI)
Gene Name
CD226
Full Name
CD226 antigen
Weight
Alternative Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1 CD226 DNAM-1, DNAM1, PTA1, TLiSA1 CD226 molecule CD226 antigen|DNAX accessory molecule-1|T lineage-specific activation antigen 1 antigen|adhesion glycoprotein|platelet and T cell activation antigen 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD226, check out the CD226 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD226: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human DNAM-1/CD226 ELISA Kit PicoKine® (EK2161)
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