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Product Info Summary
| SKU: | EK2149 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CTGF ELISA Kit PicoKine®
SKU/Catalog Number
EK2149
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CTGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTGF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CTGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2149)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q27-A349
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2149 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2149 is reactive to CCN2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2149 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCN2
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2149-CAP | Anti-Human CCN2 Pre-coated 96-well strip microplate | 1 |
| EK2149-ST | Human CCN2 Standard | 2 vials, 10 ng/tube |
| EK2149-DA | Human CCN2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.016 | 0.033 | 0.065 | 0.122 | 0.289 | 0.594 | 1.052 | 1.887 |
Data Example Images
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Human CTGF PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCN2, Dilution ratio of 1:100, concentration in serum and plasma is 10-34 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CTGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 41 | 153 | 695 | 45 | 166 | 726 |
| Standard deviation | 2.3 | 8.72 | 34.06 | 2.75 | 9.13 | 28.31 |
| CV(%) | 5.6% | 5.7% | 4.9% | 6.1% | 5.5% | 3.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCN2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 41 | 61 | 48 | 55 | 51 | 1.85 | 3.6% |
| Sample 2 | 153 | 167 | 185 | 172 | 169 | 6.77 | 4% |
| Sample 3 | 695 | 701 | 755 | 786 | 734 | 38.18 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For CCN2 (Source: Uniprot.Org, NCBI)
Gene Name
CCN2
Full Name
CCN family member 2
Weight
Superfamily
CCN family
Alternative Names
CCN family member 2 CCN2 CTGF, HCS24, IGFBP8, NOV2 cellular communication network factor 2 CCN family member 2|IBP-8|IGF-binding protein 8|IGFBP-8|connective tissue growth factor|hypertrophic chondrocyte-specific protein 24|insulin-like growth factor-binding protein 8
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCN2, check out the CCN2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CTGF ELISA Kit PicoKine® (EK2149)
Hello CJ!
EK2149 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The effect of DHEA treatment on the oxidative stress and myocardial fibrosis induced by Keshan disease pathogenic factors
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