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Product Info Summary
| SKU: | EK2182 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CRELD1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2182
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CRELD1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CRELD1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CRELD1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2182)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q30-E362
Sensitivity
<10 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2182 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2182 is reactive to CRELD1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2182 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CRELD1
Cysteine-rich with EGF-like domain protein 1 is a protein that in humans is encoded by the CRELD1 gene.This gene encodes a member of a subfamily of epidermal growth factor-related proteins. The encoded protein is characterized by a cysteine-rich with epidermal growth factor-like domain. This protein may function as a cell adhesion molecule. Mutations in this gene are the cause of atrioventricular septal defect. Alternate splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2182-CAP | Anti-Human CRELD1 Pre-coated 96-well strip microplate | 1 |
| EK2182-ST | Human CRELD1 Standard | 2 vials, 10 ng/tube |
| EK2182-DA | Human CRELD1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.041 | 0.073 | 0.182 | 0.367 | 0.623 | 0.947 | 1.524 | 2.156 |
Data Example Images
Click image to see more details
Human CRELD1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CRELD1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 91 | 368 | 952 | 92 | 349 | 925 |
| Standard deviation | 4.64 | 12.88 | 37.13 | 3.77 | 15.01 | 43.48 |
| CV(%) | 5.1% | 3.5% | 3.9% | 4.1% | 4.3% | 4.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CRELD1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 91 | 95 | 88 | 97 | 93 | 4.17 | 4.5% |
| Sample 2 | 368 | 375 | 363 | 384 | 373 | 18.25 | 4.9% |
| Sample 3 | 952 | 988 | 971 | 967 | 970 | 56.23 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For CRELD1 (Source: Uniprot.Org, NCBI)
Gene Name
CRELD1
Full Name
Protein disulfide isomerase CRELD1
Weight
45.44kDa
Superfamily
CRELD family
Alternative Names
atrioventricular septal defect 2; AVSD2; CIRRIN; CRELD1; cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213 CRELD1 AVSD2, CIRRIN cysteine rich with EGF like domains 1 protein disulfide isomerase CRELD1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CRELD1, check out the CRELD1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CRELD1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CRELD1 ELISA Kit PicoKine® (EK2182)
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