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Product Info Summary
| SKU: | EK2180 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
Product info
Product Name
Human CPB1 ELISA Kit PicoKine®
View all Carboxypeptidase B1/CPB1 ELISA kits
SKU/Catalog Number
EK2180
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CPB1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CPB1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CPB1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2180)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: H16-Y417
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2180 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2180 is reactive to CPB1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
EK2180 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carboxypeptidase B1/CPB1
Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2180-CAP | Anti-Human CPB1 Pre-coated 96-well strip microplate | 1 |
| EK2180-ST | Human CPB1 Standard | 2 vials, 20 ng/tube |
| EK2180-DA | Human CPB1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.033 | 0.069 | 0.144 | 0.295 | 0.484 | 0.976 | 1.652 | 2.379 |
Data Example Images
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Human CPB1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CPB1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 546 | 1734 | 7264 | 549 | 1753 | 7636 |
| Standard deviation | 34.4 | 104.04 | 450.37 | 34.59 | 103.43 | 465.8 |
| CV(%) | 6.3% | 6% | 6.2% | 6.3% | 5.9% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Carboxypeptidase B1/CPB1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 546 | 565 | 542 | 551 | 551 | 23.69 | 4.3% |
| Sample 2 | 1734 | 1726 | 1754 | 1771 | 1746 | 80.33 | 4.6% |
| Sample 3 | 7234 | 7302 | 7165 | 7139 | 7210 | 410.97 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For CPB1 (Source: Uniprot.Org, NCBI)
Gene Name
CPB1
Full Name
Carboxypeptidase B
Weight
47.368kDa
Superfamily
peptidase M14 family
Alternative Names
carboxypeptidase B; carboxypeptidase B1 (tissue); Carboxypeptidase B1; CPB; CPB1; DKFZp779K1333; EC 3.4.17; EC 3.4.17.2; Pancreas-specific protein; PASP; PCPB; procarboxypeptidase B CPB1 CPB, PASP, PCPB carboxypeptidase B1 carboxypeptidase B|carboxypeptidase B1 (tissue)|pancreas-specific protein|pancreatic carboxypeptidase B|procarboxypeptidase B|protaminase|tissue carboxypeptidase B
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CPB1, check out the CPB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CPB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CPB1 ELISA Kit PicoKine® (EK2180)
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