Human CLEC5A ELISA Kit PicoKine®

Product Name

Human CLEC5A ELISA Kit PicoKine®

View all MDL-1/CLEC5A ELISA kits

SKU/Catalog Number

EK2175

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CLEC5A ELISA Kit PicoKine® (96 Tests). Quantitate Human CLEC5A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CLEC5A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2175)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Y26-K188

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

See datasheet of EK2175 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2175 is reactive to CLEC5A in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2175 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MDL-1/CLEC5A

C-type lectin domain family 5 member A (CLEC5A), also known as C-type lectin superfamily member 5 (CLECSF5) and myeloid DAP12-associating lectin 1 (MDL-1) is a C-type lectin that in humans is encoded by the CLEC5A gene. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined.

Kit Components

Catalog Number	Description	Quantity
EK2175-CAP	Anti-Human CLEC5A Pre-coated 96-well strip microplate	1
EK2175-ST	Human CLEC5A Standard	2 vials, 10 ng/tube
EK2175-DA	Human CLEC5A Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.035	0.074	0.156	0.385	0.801	1.323	1.876	2.233

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CLEC5A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MDL-1/CLEC5A in ELISA kits from four different production batches/lots.

Gene/Protein Information For CLEC5A (Source: Uniprot.Org, NCBI)

Alternative Names

CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1 CLEC5A CLECSF5, MDL-1, MDL1 C-type lectin domain containing 5A C-type lectin domain family 5 member A|C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 5|C-type lectin superfamily member 5|myeloid DAP12-associating lectin-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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