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Product Info Summary
| SKU: | EK2166 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CFHR4 ELISA Kit PicoKine®
View all Complement Factor H-related 4/CFHR4 ELISA kits
SKU/Catalog Number
EK2166
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CFHR4 ELISA Kit PicoKine® (96 Tests). Quantitate Human CFHR4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CFHR4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2166)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E20-E578
Sensitivity
<20 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2166 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2166 is reactive to CFHR4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2166 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor H-related 4/CFHR4
Complement factor H-related protein 4 is a protein that in humans is encoded by the CFHR4 gene. This gene is a member of the complement factor H (CFH) gene family, and encodes one of the 5 CFH-related (CFHR) proteins. These 5 genes are closely linked to the CFH gene on chromosome 1q31-q32. The CFHRs are secreted plasma proteins synthesized primarily by the hepatocytes, and composed of highly-related short consensus repeats (SCRs). This protein enhances the cofactor activity of CFH, and is involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism. Alternatively spliced transcript variants encoding different isoforms (varying in the number of SCRs) have been described for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2166-CAP | Anti-Human CFHR4 Pre-coated 96-well strip microplate | 1 |
| EK2166-ST | Human CFHR4 Standard | 2 vials, 20 ng/tube |
| EK2166-DA | Human CFHR4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.029 | 0.072 | 0.143 | 0.302 | 0.591 | 0.961 | 1.431 | 2.167 |
Data Example Images
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Human CFHR1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CFHR4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 452 | 1663 | 4416 | 512 | 1698 | 4646 |
| Standard deviation | 23.05 | 58.21 | 172.22 | 20.99 | 73.01 | 218.36 |
| CV(%) | 5.1% | 3.5% | 3.9% | 4.1% | 4.3% | 4.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H-related 4/CFHR4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 452 | 461 | 470 | 482 | 466 | 21.45 | 4.6% |
| Sample 2 | 1663 | 1653 | 1743 | 1732 | 1698 | 54.33 | 3.2% |
| Sample 3 | 4416 | 4612 | 4741 | 4674 | 4611 | 198.26 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For CFHR4 (Source: Uniprot.Org, NCBI)
Gene Name
CFHR4
Full Name
Complement factor H-related protein 4
Weight
65.351kDa
Alternative Names
complement factor H-related protein 4; CFHL4; CFHR4; Complement Factor Hrelated 4; Complement Factor H-related 4; FHR4; FHR-4 CFHR4 CFHL4, FHR-4, FHR4 complement factor H related 4 complement factor H-related protein 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CFHR4, check out the CFHR4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFHR4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CFHR4 ELISA Kit PicoKine® (EK2166)
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