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Product Info Summary
| SKU: | EK2222 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD94 ELISA Kit PicoKine®
SKU/Catalog Number
EK2222
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD94 PicoKine ELISA Kit® (96 Tests). Quantitate Human CD94 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 24 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD94 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2222)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: K32-I179
Sensitivity
<24 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2222 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2222 is reactive to KLRD1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2222 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD94
CD94 (Cluster of Differentiation 94), also known as killer cell lectin-like receptor subfamily D, member 1 (KLRD1) is a human gene. Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Several transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2222-CAP | Anti-Human KLRD1 Pre-coated 96-well strip microplate | 1 |
| EK2222-ST | Human KLRD1 Standard | 2 vials, 10 ng/tube |
| EK2222-DA | Human KLRD1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.003 | 0.042 | 0.085 | 0.189 | 0.468 | 0.829 | 1.320 | 1.890 |
Data Example Images
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Human CD94 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD94, Dilution ratio of 1:1, concentration in serum and plasma is 583-978 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD94 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 78 | 172 | 1200 | 67 | 172 | 1212 |
| Standard deviation | 3.29 | 12.83 | 87.24 | 2.3 | 11.27 | 63.15 |
| CV(%) | 4.2% | 7.5% | 7.3% | 3.4% | 6.6% | 5.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD94 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 79 | 66 | 80 | 62 | 72 | 2.55 | 3.6% |
| Sample 2 | 125 | 148 | 150 | 179 | 151 | 10.14 | 6.7% |
| Sample 3 | 924 | 1005 | 1015 | 1430 | 1094 | 36.96 | 3.4% |
Protein Target Info & Infographic
Gene/Protein Information For KLRD1 (Source: Uniprot.Org, NCBI)
Gene Name
KLRD1
Full Name
Natural killer cells antigen CD94
Weight
20.513kDa
Alternative Names
CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor KLRD1 CD94 killer cell lectin like receptor D1 natural killer cells antigen CD94|CD94 antigen|KP43|NK cell receptor|killer cell lectin-like receptor subfamily D, member 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KLRD1, check out the KLRD1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KLRD1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD94 ELISA Kit PicoKine® (EK2222)
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