Human CD93/C1qR PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human CD93/C1qR PicoKine® Quick ELISA Kit

See all C1qR1/CD93 products

SKU/Catalog Number

FEK1320

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD93/C1qR PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CD93 in cell culture supernatants, serum, plasma ( heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD93/C1qR PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1320)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A24-K580

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK1320 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1320 is reactive to CD93 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma ( heparin, EDTA) , saliva and urine.

Application Guarantee

FEK1320 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of C1qR1/CD93

CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

Kit Components

Catalog number	Description	Quantity
FEK1320-CAP	96-well plate precoated with anti-Human C1qR1/CD93 antibody	1
EK1320-ST	lyophilized recombinant Human C1qR1/CD93 standard	10 ng/tube
FEK1320-DA	Biotinylated anti-Human C1qR1/CD93 detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.004	0.252	0.465	1.152	1.698	1.785	2.284	2.342

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD93/C1qR PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of C1qR1/CD93 in ELISA kits from four different production batches/lots.

Gene/Protein Information For CD93 (Source: Uniprot.Org, NCBI)

Alternative Names

Complement component C1q receptor;C1q/MBL/SPA receptor;C1qR;C1qR (p);C1qRp;CDw93;Complement component 1 q subcomponent receptor 1;Matrix-remodeling-associated protein 4;CD93;CD93;C1QR1, MXRA4; Cd93|6030404G09Rik, AA145088, AA4, AA4.1, AW555904, C1q, C1qr, C1qr1, C1qrp, Ly6, Ly68|CD93 |complement component C1q receptor|C1q/MBL/SPA receptor|C1qR(p)|cell surface AA4|complement component 1, q subcomponent, receptor 1|ly-68|lymphocyte 68

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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