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Product Info Summary
| SKU: | EK2151 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
Product info
Product Name
Human CD58 ELISA Kit PicoKine®
View all CD58/LFA-3 ELISA kits
SKU/Catalog Number
EK2151
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD58 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD58 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD58 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2151)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: HEK293; Immunogen sequence: F29-R215
Sensitivity
<20 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2151 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2151 is reactive to CD58 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
EK2151 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD58/LFA-3
CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2151-CAP | Anti-Human CD58 Pre-coated 96-well strip microplate | 1 |
| EK2151-ST | Human CD58 Standard | 2 vials, 20 ng/tube |
| EK2151-DA | Human CD58 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.015 | 0.036 | 0.082 | 0.165 | 0.342 | 0.712 | 1.122 | 2.151 |
Data Example Images
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Human CD58 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD58 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 285 | 1532 | 5634 | 296 | 1602 | 5648 |
| Standard deviation | 9.41 | 55.15 | 241.38 | 15.39 | 62.48 | 271.1 |
| CV(%) | 3.3% | 3.6% | 4.5% | 5.2% | 3.9% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD58/LFA-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 285 | 312 | 294 | 277 | 292 | 16.06 | 5.5% |
| Sample 2 | 1532 | 1522 | 1556 | 1649 | 1565 | 87.63 | 5.6% |
| Sample 3 | 5364 | 5841 | 5964 | 6011 | 5795 | 399.86 | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For CD58 (Source: Uniprot.Org, NCBI)
Gene Name
CD58
Full Name
Lymphocyte function-associated antigen 3
Weight
Alternative Names
Ag3; CD58 antigen; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; CD58; FLJ23181; FLJ43722; LFA3; LFA-3; LFA3ag3; lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3 CD58 LFA-3, LFA3, ag3 CD58 molecule lymphocyte function-associated antigen 3|CD58 antigen, (lymphocyte function-associated antigen 3)|surface glycoprotein LFA-3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD58, check out the CD58 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD58: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD58 ELISA Kit PicoKine® (EK2151)
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