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Product Info Summary
| SKU: | FEK1255 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CD320/8D6A PicoKine® Quick ELISA Kit
See all CD320/TCblR/8D6A products
SKU/Catalog Number
FEK1255
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD320/8D6A PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD320/8D6A in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CD320/8D6A PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1255)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S36-Y229
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1255 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1255 is reactive to CD320 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Application Guarantee
FEK1255 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD320/TCblR/8D6A
CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin(vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1255-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1255-ST | Human CD320/TCblR/8D6A Standard | 2 | 10 ng/tube |
| FEK1255-A | Human CD320 Antibody A | 1 | 3ml |
| FEK1255-B | Human CD320 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.067 | 0.179 | 0.254 | 0.444 | 0.704 | 1.245 | 1.739 | 2.243 |
Data Example Images
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Human CD320 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD320/8D6A PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 264 | 941 | 2154 | 301 | 1004 | 2278 |
| Standard deviation | 13.46 | 53.64 | 127.09 | 15.65 | 69.28 | 145.79 |
| CV(%) | 5.1% | 5.7% | 5.9% | 5.2% | 6.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD320/TCblR/8D6A in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 264 | 301 | 245 | 283 | 273 | 9.29 | 3.4% |
| Sample 2 | 941 | 882 | 1041 | 996 | 965 | 53.08 | 5.5% |
| Sample 3 | 2154 | 2260 | 2312 | 2041 | 2192 | 105.2 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For CD320 (Source: Uniprot.Org, NCBI)
Gene Name
CD320
Full Name
CD320 antigen
Weight
28.991kDa
Alternative Names
8D6 antigen; 8D6A; 8D6AFDC-SM-8D6; CD320 antigenFDC-signaling molecule 8D6,8D6TCBLR; CD320 molecule; CD320; TCblR; Transcobalamin receptor CD320 8D6, 8D6A, TCBLR, TCN2R CD320 molecule CD320 |8D6 |FDC-SM-8D6|FDC-signaling molecule 8D6|transcobalamin 2 receptor|transcobalamin receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD320, check out the CD320 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD320: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD320/8D6A PicoKine® Quick ELISA Kit (FEK1255)
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