CD320 antigen Antibodies

CD320 antigen (CD320)

Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed:18779389). Plays an essential role in cobalamin uptake (PubMed:18779389, PubMed:20524213).

Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal centre B cells (PubMed:10727470). Functions as costimulator to promote B cell responses to antigenic stimulation; promotes B cell differentiation and proliferation (PubMed:10727470, PubMed:11418631).

Gene Information

Human
Gene Name:	CD320
Uniprot:	Q9NPF0
Entrez:	51293

Family

Belongs to:
No superfamily

Alternative Names

8D6 antigen; 8D6A; 8D6AFDC-SM-8D6; CD320 antigenFDC-signaling molecule 8D6,8D6TCBLR; CD320 molecule; CD320; TCblR; Transcobalamin receptor

Molecular Weight

Mass (kDA):
28.991 kDA

Genomic Context

Human
Location:	19p13.2
Sequence:	19; NC_000019.10 (8302127..8308358, complement)

Tissue Specificity

Detected in the germinal center (GC) of lymphoid follicles (at protein level) (PubMed:11418631). Expressed abundantly on follicular dendritic cells (FDCs) (PubMed:10727470).

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein.

Diseases

• Malignant Neoplasms
• Acquired Immunodeficiency Syndrome
• Adenocarcinoma
• Neoplasms
• Colorectal Neoplasms
• Nervousness
• Inborn Errors Of Metabolism
• Hiv Infections

• Tissue Adhesions
• Methylmalonic Acidemia
• Vitamin B 12 Deficiency

Pathways

• Cell Adhesion
• Methylation
• Transport
• Cell Division
• Cell Proliferation
• Dna Hypomethylation
• Cell Cycle
• Dna Methylation

• Neurogenesis
• Endocytosis

PTMs

• Biotinylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CD320

Best Uses Of The CD320 Marker

The CD320 is an expression site for human proteins. A LDL receptor motif regulates its role in cell membrane targeting. This protein has clinical applications in animal studies and in human medicine. It is not only found in human cells but also in immune system cells. Here are some uses and how you can find out if this protein is present in human cells.

CD320: The molecular structure of CD320

Molecular structure of human CD320 marker. A gene with this structure is expressed in most human tissues. It has been implicated previously in the development neural tube defects. This gene was also associated with a lower uptake of vitamin B12 when cultured in cell cultures. This paper describes a novel approach to the identification of this marker. It is based in the cell cycle dependence CD320 marker.

Regulation of membrane targeting via LDL receptor motif

The hepatic GALE protein has a role in cholesterol content control, HDL catabolism, and whole-body glucose homeostasis. It has been shown to regulate membrane targeting of the LDL receptor. This study highlights the role of the hepatic GALEprotein in cholesterol content control. It also demonstrates the importance of Apo AI (PPARa) in lipid metabolism.

The HNF4a, LXR, and LXR genes both act in tandem. Both activate the promoters for genes involved in lipolysis. However, their interactions inhibit the induction of lipogenic genes by the liver X receptor (LDLR). Both LXR and GR are implicated in early resistance to HFHS-induced fatty liver. It is not clear if these two molecules mediate positive feedback regulation of LDL receptors during hepatic Steatosis.

The proposed nomenclature was based on a consensus-driven analysis of multiple scientific papers. Acta Pharm Sin B features Finck BN and Lu H discussing the role played by the LDL receptor motif during lipoprotein metabolism. LDL cholesterol in the liver is decreased when LDL receptor motif expression is inhibited. The article also mentions the role of Lipin-1, an inducible amplifier that regulates fatty acid flux.

Clinical applications

The CD320 marker appears on the cell surfaces in 5i/L/FA cultures of naive and epiblast cells. CD320 is not a component of naive hPSC reprogramming. It is a transcobalamin receptor, which mediates the uptake of vitamin B12.

The CD320 genes are found in the human genome. They have the UniProt Code Q9NPF0. Its UniProt is Q9NPF0. It could guide clinical action in patients suffering from pancreatic cancer. Its use in clinical practice is not yet widespread. Patients with pancreatic cancer have a very low median survival rate. The CD320 marker could be used clinically to increase the patient's survival rate.

Cell surface markers are increasingly used for studying human pluripotent states. However, many cell types and subtypes remain unidentified. CD320 is often used in immunological research, but it is not widely used in clinical settings. In the meantime, researchers can use it to separate advanced reprogramming cells from naive cells. A study is being done to determine the function of CD24 in naive HPSC.

ARE-mediated sorting

A transmembrane protein marker like CD320 is necessary for the ARE-mediated sorting proteins. In addition to CD320, other markers are required for sorting. The CD320 protein has a cytoplasmic tail that contains the transmembrane signal. Sorting is affected by the protein’s glycan and saccharides. Modifications in the structure of these molecules could regulate protein targeting.

Glycans play an important role in epithelial function, and may also be involved in glycan sorting. N-glycans have been implicated in many cell types' studies. Moreover, these glycans are diverse, with different oligosaccharides attached to a protein. Furthermore, these glycans are specific to cell types and developmental stages, which suggests that the ARE-mediated sorting mechanism of cells may be biochemically linked.

Immunohistochemical staining

It is possible to determine the presence of a specific protein marker by immunohistochemical staining using the CD-320 marker. These protein markers may be useful in diagnosing and classifying cancers. The basic steps of the staining process are outlined in this guide. You can also apply for continuing education credits if you have already viewed this educational material. We hope this guide will help you get started.

The antibody is available in two forms: RTU concentrated and RTU pre-diluted. Concentrate is flexible, cheaper, and can be used for any staining system, whether it's automated or manual. To balance cost and staining duration, you can adjust your staining system's antibody concentration. The antigens will lose their staining power with time and exposure to the air.

The CD320 marker, also known as 8D6 and 8D6A, is a transmembrane glycoprotein that is abundantly expressed on follicular dendritic cells. It has been shown to enhance the proliferation of germinal center B cells and is also a receptor for transcobalamin. Mutations in CD320 lead to methylmalonic Aciduria type (TCblR). It is important not to forget that CD320 antibody detects bands measuring 58-70 kDa. However statistically, this difference does not matter.

Molecular biology

The CD320 molecule encodes a transcobalamin receptor. This receptor is expressed on cells' surface and is a novel antagonist of cytokine receptors with therapeutic potential in treatment of autoimmune conditions. Other diseases that are associated with CD320 include methylmalonic acidsuria and methylmalonic alemia. CD320 is easily measured in serum, plasma, or tissue samples using the Assay Genie Human CD320 ELISA.

Human CD320 consists mainly of an N–terminal extracellular zone, two LDLR and one EGF modules, as well as a transmembrane TM (TM) domain. The LDLR1 motif contains a DGSDE element, while the LDLR2 motif has a DSSDE element. Human kidney sections stained with a blue dye show CD320 expressed on proximal tubules.

Both Corin & CD320 are cell surface receptors. These receptors are transported from Golgi by central recycling endosomes. Both markers are recognized by the Rab11a sorting station and may undergo re-sorting. Mutations that alter the DSSDE motif in one protein cause apical transcription. It is important that both CD320 (and corin) are present in cells.

Using the CD320 marker in Molecular Biology, researchers can identify different types and types of cells by comparing the gene expression levels. This gene expression level correlates with the number of cancerous cells in a given tissue. This information can also be useful for finding the genetic basis of disease. MtDNA contains many cytochrome genes. Gene information and antibodies can be used to analyze the expression of these genes in tumor cells.